Anti-BCL6 Antibody Picoband™

Boster Bio Anti-BCL6 Antibody Picoband™ catalog # A00142-1. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00142-1
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, ICC, WB

Product Name

Anti-BCL6 Antibody Picoband™

See all Bcl-6 products

SKU/Catalog Number

A00142-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BCL6 Antibody Picoband™ catalog # A00142-1. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BCL6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00142-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence of human BCL6 (ASPADSCIQFTRHASDVLLNLNRLRSRDILTD).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00142-1 is reactive to BCL6 in Human, Mouse, Rat

Applications

A00142-1 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For BCL6 (Source: Uniprot.Org, NCBI)

Uniprot ID

P41182

Gene ID

604

Gene Name

BCL6

Full Name

B-cell lymphoma 6 protein

Weight

78.846kDa

Alternative Names

B-cell CLL/lymphoma 6; B-cell lymphoma 6 protein transcript; Bcl-5; BCL5B-cell lymphoma 5 protein; Bcl6; Bcl-6; BCL6A; cys-his2 zinc finger transcription factor; LAZ3; LAZ3ZBTB27B-cell lymphoma 6 protein; lymphoma-associated zinc finger gene on chromosome 3; Protein LAZ-3; ZBTB27 ; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51ZNF51; zinc finger transcription factor BCL6S; ZNF51 BCL6 BCL5A, LAZ3, ZBTB27, ZNF51, BCL6 BCL6 transcription repressor B-cell lymphoma 6 protein|B cell CLL/lymphoma 6|B-cell lymphoma 5 protein|B-cell lymphoma 6 protein transcript|BCL-5|BCL-6|cys-his2 zinc finger transcription factor|lymphoma-associated zinc finger gene on chromosome 3|protein LAZ-3|zinc finger and BTB domain-containing protein 27|zinc finger protein 51|zinc finger transcription factor BCL6S

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BCL6, check out the BCL6 Infographic

BCL6 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for BCL6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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16 Customer Q&As for Anti-BCL6 Antibody Picoband™

Question

Will anti-BCL6 antibody A00142-1 work for ICC with urinary bladder?

Verified Customer

Verified customer

Asked: 2020-05-06

Answer

According to the expression profile of urinary bladder, BCL6 is highly expressed in urinary bladder. So, it is likely that anti-BCL6 antibody A00142-1 will work for ICC with urinary bladder.

Boster Scientific Support

Answered: 2020-05-06

Question

Here is the WB image, lot number and protocol we used for urinary bladder using anti-BCL6 antibody A00142-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-27

Question

We have observed staining in mouse cervix carcinoma. Are there any suggestions? Is anti-BCL6 antibody supposed to stain cervix carcinoma positively?

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

According to literature cervix carcinoma does express BCL6. According to Uniprot.org, BCL6 is expressed in urinary bladder, skeletal muscle, liver, cervix carcinoma, among other tissues. Regarding which tissues have BCL6 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Liver, Pubmed ID: 8274740, 8506375
Skeletal muscle, Pubmed ID: 8220427

Boster Scientific Support

Answered: 2020-01-03

Question

We are interested in using your anti-BCL6 antibody for negative regulation of cellular senescence studies. Has this antibody been tested with western blotting on rat spleen tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-12

Answer

I appreciate your inquiry. This A00142-1 anti-BCL6 antibody is tested on human placenta tissue, raji whole cell lysate, tissue lysate, a549 whole cell lysate, rat spleen tissue, testis tissue, brain tissue, mouse spleen tissue, brl cells. It is guaranteed to work for Flow Cytometry, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-12

Question

Our lab were content with the WB result of your anti-BCL6 antibody . However we have been able to see positive staining in cervix carcinoma nucleus using this antibody. Is that expected? Could you tell me where is BCL6 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-05

Answer

From literature, cervix carcinoma does express BCL6. Generally BCL6 expresses in nucleus. Regarding which tissues have BCL6 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Liver, Pubmed ID: 8274740, 8506375
Skeletal muscle, Pubmed ID: 8220427

Boster Scientific Support

Answered: 2019-11-05

Question

I am interested in to test anti-BCL6 antibody A00142-1 on human urinary bladder for research purposes, then I may be interested in using anti-BCL6 antibody A00142-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-22

Answer

The products we sell, including anti-BCL6 antibody A00142-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-22

Question

Will A00142-1 anti-BCL6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-04

Answer

You can see on the product datasheet, A00142-1 anti-BCL6 antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-04

Question

I was wanting to use your anti-BCL6 antibody for ICC for human urinary bladder on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human urinary bladder identification?

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

As indicated on the product datasheet, A00142-1 anti-BCL6 antibody has been tested for Flow Cytometry, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human urinary bladder in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-16

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for urinary bladder using anti-BCL6 antibody A00142-1. Let me know if you need anything else.

V. Wu

Verified customer

Asked: 2019-07-01

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-01

Question

Can you help my question with product A00142-1, anti-BCL6 antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00142-1 anti-BCL6 antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-18

Question

Is a blocking peptide available for product anti-BCL6 antibody (A00142-1)?

Verified Customer

Verified customer

Asked: 2019-04-30

Answer

We do provide the blocking peptide for product anti-BCL6 antibody (A00142-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-04-30

Question

I see that the anti-BCL6 antibody A00142-1 works with ICC, what is the protocol used to produce the result images on the product page?

B. Bhatt

Verified customer

Asked: 2018-06-04

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-06-04

Question

Do you have a BSA free version of anti-BCL6 antibody A00142-1 available?

Verified Customer

Verified customer

Asked: 2017-10-24

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-BCL6 antibody A00142-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-10-24

Question

We are currently using anti-BCL6 antibody A00142-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?

D. Jha

Verified customer

Asked: 2016-04-25

Answer

The anti-BCL6 antibody (A00142-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-04-25

Question

We ordered your anti-BCL6 antibody for WB on urinary bladder in the past. I am using rat, and I plan to use the antibody for ICC next. I was wanting to use examining urinary bladder as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?

H. Zhao

Verified customer

Asked: 2015-07-13

Answer

I have checked the website and datasheets of our anti-BCL6 antibody and I see that A00142-1 has been tested on rat in both WB and ICC. Thus A00142-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2015-07-13

Question

Is this A00142-1 anti-BCL6 antibody reactive to the isotypes of BCL6?

M. Thomas

Verified customer

Asked: 2014-11-26

Answer

The immunogen of A00142-1 anti-BCL6 antibody is A synthetic peptide corresponding to a sequence of human BCL6 (ASPADSCIQFTRHASDVLLNLNRLRSRDILTD). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-11-26

Conjugation

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Ships directly to USA and Canada. For other regions, visit our distributors page

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A00142-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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