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Product Info Summary
| SKU: | A05905-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-Bcl9L Antibody
SKU/Catalog Number
A05905-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Bcl9L Antibody (Catalog # A05905-1). Tested in ELISA, WB, IHC-P, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
Bcl9L antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Bcl9L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05905-1)
Host
Rabbit
Contents
Bcl9L Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
Bcl9L antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human Bcl9L. The immunogen is located within amino acids 20 - 70 of Bcl9L.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Bcl9L antibody is predicted to not cross-react with other Bcl family members. At least four isoforms of Bcl9L are known to exist; this antibody will detect all four.
Reactive Species
A05905-1 is reactive to Bcl9l in Human
Applications
A05905-1 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
156.68kDa
Background of BCL9-2
Bcl9L, a homolog of Bcl9, was initially identified through a bioinformatics screening. It is expressed in fetal brain, adult lung, eye and prostate, in addition to several types of tumors including pancreatic and prostate cancers. Bcl9L has been shown to interact with beta-catenin, a target of the Wnt signaling pathway, and is required for enhanced beta-catenin-T-cell factor (TCF)-mediated transcription in colorectal tumor cells, possibly by translocating beta-catenin to the nucleus. Other studies have indicated that Bcl9L expression correlates with high nuclear grade cancer phenotype and the expression of ErbB2/HER-2 in breast cancers, suggesting that activity may occur in other types of cancer. Bcl9L has also been shown to be critical for Wnt-mediate regulation of stem cell traits in colon epithelium and adenocarcinomas which are associated with tumor invasion, metastasis, and resistance to therapy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Bcl9L antibody can be used for detection of Bcl9L by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Bcl9L in HeLa cell lysate with Bcl9L antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of Bcl9L in HeLa cells with Bcl9L antibody at 10 μg/mL.
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Immunofluorescence of Bcl9L in human breast tissue with Bcl9L antibody at 20 μg/ml.
Green: Bcl9L Antibody (A05905-1)
Blue: DAPI staining
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Immunohistochemistry of Bcl9L in human breast tissue with Bcl9L antibody at 5 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For Bcl9l (Source: Uniprot.org, NCBI)
Gene Name
Bcl9l
Full Name
B-cell CLL/lymphoma 9-like protein
Weight
156.68kDa
Superfamily
BCL9 family
Alternative Names
B-cell CLL/lymphoma 9-like protein; B-cell CLL/lymphoma 9-like; B-cell lymphoma 9-like protein; Bcl92; Bcl9-2; BCL9-like protein; DLNB11; DLNB11BCL9-2; nuclear co-factor of beta-catenin signalling; Protein BCL9-2 Bcl9l|B9L, BC003321, BCL9-2, DLNB, DLNB11|B cell CLL/lymphoma 9-like|B-cell CLL/lymphoma 9-like protein|B-cell lymphoma 9-like protein|BCL9-like protein|BCL9-related beta-catenin-binding protein|nuclear co-factor of beta-catenin signalling|protein BCL9-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Bcl9l, check out the Bcl9l Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Bcl9l: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bcl9L Antibody (A05905-1)
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