Anti-BDNF Rabbit Monoclonal Antibody

Product Name

Anti-BDNF Rabbit Monoclonal Antibody

View all Bdnf Antibodies

SKU/Catalog Number

M00035-2

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-BDNF Rabbit Monoclonal Antibody catalog # M00035-2. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BDNF Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00035-2)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

17B21

Isotype

IgG

Immunogen

A synthesized peptide derived from human BDNF

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00035-2 is reactive to BDNF in Human, Mouse, Rat

Applications

M00035-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

110 kDa

Calculated molecular weight

27.818kDa

Background of Bdnf

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Click image to see more details

All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.

Click image to see more details

Western blot analysis of extracts of Mouse heart lysate, using BDNF antibody

Click image to see more details

Immunohistochemical analysis of paraffin-embedded Human breast cancer, using the Antibody at 1:1500 dilution.

Click image to see more details

Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody at 1:1500 dilution.

Click image to see more details

Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:1500 dilution.

Click image to see more details

Immunohistochemical analysis of paraffin-embedded human brain, using BDNF Antibody.

Click image to see more details

Immunofluorescent analysis using the Antibody at 1:150 dilution.

Click image to see more details

Immunofluorescent analysis using the Antibody at 1:500 dilution.

Click image to see more details

Immunofluorescent analysis of Hela cells, using BDNF Antibody.

Gene/Protein Information For BDNF (Source: Uniprot.org, NCBI)

Alternative Names

Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin BDNF ANON2, BULN2 brain derived neurotrophic factor brain-derived neurotrophic factor|abrineurin|neurotrophin

For more info on BDNF, check out the BDNF Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BDNF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the BDNF Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis