Anti-beta II Tubulin Antibody

Product Name

Anti-beta II Tubulin Antibody

View all beta II Tubulin A Antibodies

SKU/Catalog Number

M06868-1

Size

50 µl

Description

Boster Bio Anti-beta II Tubulin Antibody (Catalog # M06868-1). Tested in WB, Flow Cytometry, IF application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-beta II Tubulin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06868-1)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 14-46 amino acids from human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M06868-1 is reactive to TUBB2A in Human, Mouse, Rat

Applications

M06868-1 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

49.907kDa

Background of beta II Tubulin A

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:25
WB: 1:8000
FC: 1:25

Validation Images & Assay Conditions

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 (mouse myoblast cell line) cells labeling beta II Tubulin with M06868-1 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on C2C12 cell line. The nuclear counter stain is DAPI (blue).

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All lanes : Anti-beta II Tubulin at 1:8000 dilution
Lane 1: mouse brain lysate
Lane 2: Hela whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: PC-12 whole cell lysate
Lane 5: NIH/3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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Overlay histogram showing NIH/3T3 cells stained with M06868-1 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M06868-1, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Gene/Protein Information For TUBB2A (Source: Uniprot.org, NCBI)

Alternative Names

dJ40E16.7; TUBB; TUBB2; TUBB2B; tubulin beta-2A chain; tubulin, beta 2; tubulin, beta 2A; tubulin, beta polypeptide 2; tubulin, beta polypeptide TUBB2A CDCBM5, TUBB, TUBB2 tubulin beta 2A class IIa tubulin beta-2A chain|class IIa beta-tubulin|tubulin, beta 2A|tubulin, beta polypeptide 2

For more info on TUBB2A, check out the TUBB2A Infographic

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In this infographic, you will see the following information for TUBB2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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