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Product Info Summary
| SKU: | M01857 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01857
BM3880 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody catalog # M01857. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01857)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CB-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human beta III Tubulin
Reactive Species
M01857 is reactive to TUBB3 in Human, Mouse, Rat
Observed Molecular Weight
50 kDa
Calculated molecular weight
50.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01857 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000-1:20000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: HeLa cell
IHC: human ovarian cancer tissue
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using beta III Tubulin Antibody(M01857)
TUBB3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TUBB3 Antibody (M01857)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Western blot analysis of beta III Tubulin expression in HeLa cell lysate (M01857).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBB3 monoclonal antibody (Catalog # M01857) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBB3
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AB0042Immunofluorescent analysis of PC12 cells, using beta Tubulin Antibody .
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Specific Publications For Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody (M01857)
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody
Question
Is M01857 suitable for mouse tissue immunostaining?
Verified customer
Asked: 2019-10-09
Answer
Yes, the Anti-Beta III Tubulin TUBB3 Rabbit Monoclonal Antibody (M01857) is suitable for mouse tissue immunostaining.
Boster Scientific Support
Answered: 2019-10-09
