Anti-BLK Antibody Picoband™

Product Name

Anti-BLK Antibody Picoband™

View all BLK Antibodies

SKU/Catalog Number

A01539-4

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BLK Antibody Picoband™ catalog # A01539-4. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-BLK Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01539-4)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human BLK recombinant protein (Position: D53-E184).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01539-4 is reactive to BLK in Human

Applications

A01539-4 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

57.706kDa

Background of BLK

Tyrosine-protein kinase BLK, also known as B lymphocyte kinase, is a non-receptor tyrosine kinase that in humans is encoded by the BLK gene. This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of BLK using anti-BLK antibody (A01539-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BLK antigen affinity purified polyclonal antibody (Catalog # A01539-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BLK at approximately 60 kDa. The expected band size for BLK is at 60 kDa.

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Figure 2. Flow Cytometry analysis of Daudi cells using anti-BLK antibody (A01539-4).
Overlay histogram showing Daudi cells stained with A01539-4 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BLK Antibody (A01539-4, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For BLK (Source: Uniprot.org, NCBI)

Alternative Names

B lymphocyte kinase; B lymphoid tyrosine kinase; BLK nonreceptor tyrosine kinase; Blk; EC 2.7.10.2; MGC10442; MODY11; p55-Blk; tyrosine-protein kinase Blk BLK MODY11 BLK proto-oncogene, Src family tyrosine kinase tyrosine-protein kinase Blk|B lymphoid tyrosine kinase|BLK nonreceptor tyrosine kinase|b lymphocyte kinase|p55-Blk

For more info on BLK, check out the BLK Infographic

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In this infographic, you will see the following information for BLK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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