Anti-BMAL1/ARNTL Antibody Picoband™

Product Name

Anti-BMAL1/ARNTL Antibody Picoband™

View all BMAL1 Antibodies

SKU/Catalog Number

A00260-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BMAL1/ARNTL Antibody Picoband™ catalog # A00260-3. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BMAL1/ARNTL Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00260-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human BMAL1/ARNTL recombinant protein (Position: R116-E447).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00260-3 is reactive to ARNTL in Human, Mouse, Rat

Applications

A00260-3 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

68.762kDa

Background of BMAL1

Aryl hydrocarbon receptor nuclear translocator-like protein 1 is protein that in humans is encoded by the ARNTL gene. The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of BMAL1/ARNTL using anti-BMAL1/ARNTL antibody (A00260-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human HT1080 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse heart tissue lysates,
Lane 9: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMAL1/ARNTL antigen affinity purified polyclonal antibody (Catalog # A00260-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMAL1/ARNTL at approximately 75 kDa. The expected band size for BMAL1/ARNTL is at 69 kDa.

Gene/Protein Information For ARNTL (Source: Uniprot.org, NCBI)

Alternative Names

aryl hydrocarbon receptor nuclear translocator-like protein 1; aryl hydrocarbon receptor nuclear translocator-like; basic-helix-loop-helix-PAS orphan MOP3; BHLHE5; bHLHe5brain and muscle; bHLH-PAS protein JAP3; BMAL1TIC; Brain and muscle ARNT-like 1; Class E basic helix-loop-helix protein 5; Member of PAS protein 3; member of PAS superfamily 3; MOP3BMAL1c; PAS domain-containing protein 3; PASD3MGC47515 ARNTL BMAL1, BMAL1c, JAP3, MOP3, PASD3, TIC, bHLHe5 aryl hydrocarbon receptor nuclear translocator like aryl hydrocarbon receptor nuclear translocator-like protein 1|ARNT-like protein 1, brain and muscle|PAS domain containing 3|PAS domain-containing protein 3|bHLH-PAS protein JAP3|basic helix-loop-helix family member e5|basic-helix-loop-helix-PAS orphan MOP3|basic-helix-loop-helix-PAS protein MOP3|brain and muscle ARNT-like 1|class E basic helix-loop-helix protein 5|member of PAS protein 3|member of PAS superfamily 3|testis tissue sperm-binding protein Li 50e

For more info on ARNTL, check out the ARNTL Infographic

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In this infographic, you will see the following information for ARNTL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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