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Product Info Summary
| SKU: | PA2017 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-BMPR1B Antibody Picoband®
SKU/Catalog Number
PA2017
BA3305 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BMPR1B Antibody catalog # PA2017. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BMPR1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2017)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human BMPR1B, different from the related rat and mouse sequences by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2017 is reactive to BMPR1B in Human, Mouse, Rat
Observed Molecular Weight
22 kDa
Calculated molecular weight
56.9 kDa
Background of BMPR1B
BMPR1B (Bone Morphogenetic Protein Receptor Type IB), also known as ALK6, is a protein which in humans is encoded by the BMPR1B gene. BMPR1B is a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. By analysis of a monochromosome hybrid mapping panel and by FISH, Astrom et al. (1999) mapped the BMPR1B gene to chromosome 4q22-q24. Ide et al. (1997) compared BMP receptor expression in normal and cancerous prostate tissues. While BMPR1A and BMPR2 were expressed at similar levels in all prostate tissues, BMPR1B was expressed at a significantly reduced level in cancerous prostate tissue.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2017 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
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Anti-BMPR1B antibody, PA2017, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human BMPR1B, 49.0KD
(162aa tag+D17-D289)
Lane 1: Recombinant Human BMPR1B Protein 10ng
Lane 2: Recombinant Human BMPR1B Protein 5ng
Lane 3: Recombinant Human BMPR1B Protein 2.5ng
Lane 4: Recombinant Human BMPR1B Protein 1.25ng
Specific Publications For Anti-BMPR1B Antibody Picoband® (PA2017)
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Customer Q&As
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3 Customer Q&As for Anti-BMPR1B Antibody Picoband®
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain uterus using anti-BMPR1B antibody PA2017. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-18
Question
My question regarding product PA2017, anti-BMPR1B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2017 anti-BMPR1B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-27
Question
I see that the anti-BMPR1B antibody PA2017 works with WB, what is the protocol used to produce the result images on the product page?
S. Williams
Verified customer
Asked: 2015-06-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-06-18
