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Product Info Summary
| SKU: | M01469 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-BNIP3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01469
BM5039 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-BNIP3 Rabbit Monoclonal Antibody catalog # M01469. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BNIP3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01469)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAEI-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human BNIP3
Reactive Species
M01469 is reactive to BNIP3 in Human, Mouse, Rat
Observed Molecular Weight
30, 50 kDa
Calculated molecular weight
27.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01469 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: Human pancreatic cancer tissue, Human placenta tissue, Human thyroid cancer tissue, human kidney tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of BNIP3 expression in (1) Jurkat cell lysate; (2) Mouse spleen lysate.
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Immunohistochemical analysis of paraffin-embedded Human pancreatic cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human placenta, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded human kidney, using BNIP3 Antibody.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
Specific Publications For Anti-BNIP3 Rabbit Monoclonal Antibody (M01469)
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3 Customer Q&As for Anti-BNIP3 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-BNIP3 Rabbit Monoclonal antibody (M01469)?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
We do provide the blocking peptide for product anti-BNIP3 Rabbit Monoclonal antibody (M01469). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-20
Question
I was wanting to use your anti-BNIP3 Rabbit Monoclonal antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-01-24
Answer
As indicated on the product datasheet, M01469 anti-BNIP3 Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-24
Question
Would M01469 anti-BNIP3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Thomas
Verified customer
Asked: 2015-03-05
Answer
You can see on the product datasheet, M01469 anti-BNIP3 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-03-05
