Product Info Summary
SKU: | A08515 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-BRWD3 Antibody
SKU/Catalog Number
A08515
Size
100ul
Form
Liquid
Description
Boster Bio Anti-BRWD3 Antibody (Catalog# A08515). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BRWD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08515)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human BRWD3. Immunogen sequence location: 1751-1800
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08515 is reactive to BRWD3 in Human, Mouse
Applications
A08515 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
203.598kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from COLO cells, using BRWD3 Antibody. The lane on the right is blocked with the synthesized peptide.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human heart tissue, using BRWD3 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For BRWD3 (Source: Uniprot.org, NCBI)
Gene Name
BRWD3
Full Name
Bromodomain and WD repeat-containing protein 3
Weight
203.598kDa
Alternative Names
Bromodomain and WD repeat-containing protein 3 BRWD3 BRODL, MRX93 bromodomain and WD repeat domain containing 3 bromodomain and WD repeat-containing protein 3|bromo domain-containing protein disrupted in leukemia|novel WD repeat domain protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BRWD3, check out the BRWD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BRWD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BRWD3 Antibody (A08515)
Hello CJ!
No publications found for A08515
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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4 Customer Q&As for Anti-BRWD3 Antibody
Question
Is this A08515 anti-BRWD3 antibody reactive to the isotypes of BRWD3?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
The immunogen of A08515 anti-BRWD3 antibody is A peptide derived from human BRWD3. Immunogen sequence location: 1751-1800. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-11
Question
We are currently using anti-BRWD3 antibody A08515 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
The anti-BRWD3 antibody (A08515) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-24
Question
I was wanting to use to test anti-BRWD3 antibody A08515 on human cervix carcinoma for research purposes, then I may be interested in using anti-BRWD3 antibody A08515 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Krishna
Verified customer
Asked: 2017-03-14
Answer
The products we sell, including anti-BRWD3 antibody A08515, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-03-14
Question
I have a question about product A08515, anti-BRWD3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Evans
Verified customer
Asked: 2013-01-29
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08515 anti-BRWD3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-01-29