Anti-BTN3A1/2/3 Antibody

Boster Bio Anti-BTN3A1/2/3 Antibody catalog # A04257. Tested in IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: A04257
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: IHC

Product Name

Anti-BTN3A1/2/3 Antibody

See all CD277/BTN3A1 products

SKU/Catalog Number

A04257

Size

100μl

Form

Liquid

Description

Boster Bio Anti-BTN3A1/2/3 Antibody catalog # A04257. Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BTN3A1/2/3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04257)

Host

Rabbit

Contents

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Isotype

IgG

Immunogen

Synthetic peptide from human protein at AA range: 41-90.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04257 is reactive to BTN3A1 in Human

Applications

A04257 is guaranteed for IHC Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

IHC-P, 1:50-300

Validation Images & Assay Conditions

Gene/Protein Information For BTN3A1 (Source: Uniprot.Org, NCBI)

Uniprot ID

O00481

Gene ID

11119

Gene Name

BTN3A1

Full Name

Butyrophilin subfamily 3 member A1

Weight

57.677kDa

Superfamily

immunoglobulin superfamily

Alternative Names

BT3.1; BTF5; BTN3A1; butyrophilin, subfamily 3, member A1; CD277; dJ45P21.3 (butyrophilin, subfamily 3, member A1) BTN3A1 BT3.1, BTF5, BTN3.1, CD277 butyrophilin subfamily 3 member A1 butyrophilin subfamily 3 member A1|dJ45P21.3 (butyrophilin, subfamily 3, member A1)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BTN3A1, check out the BTN3A1 Infographic

BTN3A1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for BTN3A1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A04257

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13 Customer Q&As for Anti-BTN3A1/2/3 Antibody

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for hippocampus thalamus using anti-BTN3A1/2/3 antibody A04257. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-04-07

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-07

Question

Does anti-BTN3A1/2/3 antibody A04257 work for IHC with hippocampus thalamus?

Verified Customer

Verified customer

Asked: 2020-02-27

Answer

According to the expression profile of hippocampus thalamus, BTN3A1 is highly expressed in hippocampus thalamus. So, it is likely that anti-BTN3A1/2/3 antibody A04257 will work for IHC with hippocampus thalamus.

Boster Scientific Support

Answered: 2020-02-27

Question

We are currently using anti-BTN3A1/2/3 antibody A04257 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?

J. Miller

Verified customer

Asked: 2019-12-17

Answer

The anti-BTN3A1/2/3 antibody (A04257) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-17

Question

My lab would like to test anti-BTN3A1/2/3 antibody A04257 on human hippocampus thalamus for research purposes, then I may be interested in using anti-BTN3A1/2/3 antibody A04257 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

The products we sell, including anti-BTN3A1/2/3 antibody A04257, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-13

Question

I was wanting to use your anti-BTN3A1/2/3 antibody for IHC for human hippocampus thalamus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human hippocampus thalamus identification?

Verified Customer

Verified customer

Asked: 2019-10-28

Answer

It shows on the product datasheet, A04257 anti-BTN3A1/2/3 antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human hippocampus thalamus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-28

Question

I see that the anti-BTN3A1/2/3 antibody A04257 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-10

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-10

Question

Would anti-BTN3A1/2/3 antibody A04257 work on goat IHC with small intestine peyer's patch?

Verified Customer

Verified customer

Asked: 2019-05-31

Answer

Our lab technicians have not tested anti-BTN3A1/2/3 antibody A04257 on goat. You can run a BLAST between goat and the immunogen sequence of anti-BTN3A1/2/3 antibody A04257 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat small intestine peyer's patch in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-31

Question

Is there a BSA free version of anti-BTN3A1/2/3 antibody A04257 available?

Verified Customer

Verified customer

Asked: 2019-01-23

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-BTN3A1/2/3 antibody A04257 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-01-23

Question

My question regarding product A04257, anti-BTN3A1/2/3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-12-07

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04257 anti-BTN3A1/2/3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-12-07

Question

Is this A04257 anti-BTN3A1/2/3 antibody reactive to the isotypes of BTN3A1?

H. Jones

Verified customer

Asked: 2017-06-21

Answer

The immunogen of A04257 anti-BTN3A1/2/3 antibody is Synthetic peptide from human protein at AA range: 41-90. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-06-21

Question

Is a blocking peptide available for product anti-BTN3A1/2/3 antibody (A04257)?

Verified Customer

Verified customer

Asked: 2017-06-21

Answer

We do provide the blocking peptide for product anti-BTN3A1/2/3 antibody (A04257). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-06-21

Question

Will A04257 anti-BTN3A1/2/3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-05-25

Answer

As indicated on the product datasheet, A04257 anti-BTN3A1/2/3 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-05-25

Question

Here is the WB image, lot number and protocol we used for hippocampus thalamus using anti-BTN3A1/2/3 antibody A04257. Please let me know if you require anything else.

S. Parker

Verified customer

Asked: 2014-04-14

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-04-14

Size

Total: $299

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A04257
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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