Anti-C-FOS Monoclonal Antibody

Boster Bio Anti-C-FOS Monoclonal Antibody catalog # M00297-3. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 5 publication(s).

Product Info Summary

SKU: M00297-3
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: IF, IHC, ICC, WB

Product Name

Anti-C-FOS Monoclonal Antibody

See all c-Fos products

SKU/Catalog Number

M00297-3

Size

100ul

Description

Boster Bio Anti-C-FOS Monoclonal Antibody catalog # M00297-3. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-C-FOS Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00297-3)

Host

Mouse

Contents

Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3

Clonality

Monoclonal

Immunogen

Full length recombinant human protein expressed in and purified from E. coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00297-3 is reactive to FOS in Human, Mouse, Rat

Applications

M00297-3 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB: 1:1,000-2,000, IF/ICC or IHC: 1:1,000

Validation Images & Assay Conditions

Gene/Protein Information For FOS (Source: Uniprot.Org, NCBI)

Uniprot ID

P01100

Gene ID

2353

Gene Name

FOS

Full Name

Proto-oncogene c-Fos

Weight

40.695kDa

Superfamily

bZIP family

Alternative Names

activator protein 1; AP-1; cellular oncogene c-fos; Cellular oncogene fos; cFos; c-Fos; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); FBJ murine osteosarcoma viral oncogene homolog; FOS; G0/G1 switch regulatory protein 7; G0S7; proto-oncogene c-Fos; v-fos FBJ murine osteosarcoma viral oncogene homolog FOS AP-1, C-FOS, p55 Fos proto-oncogene, AP-1 transcription factor subunit proto-oncogene c-Fos|FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)|FBJ murine osteosarcoma viral oncogene homolog|Fos proto-oncogene, AP-1 trancription factor subunit|G0/G1 switch regulatory protein 7|activator protein 1|cellular oncogene c-fos

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FOS, check out the FOS Infographic

FOS infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FOS: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00297-3 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Overexpression of c-fos in Helicobacter pylori-induced gastric precancerosis of Mongolian gerbil

Changes in the expression of c-fos & heat shock protein genes & blood flow velocity in the brain of rats undergoing myocardial ischaemia/reperfusion

Analgesic Neural Circuits Are Activated by Electroacupuncture at Two Sets of Acupoints

miR-144 and targets, c-fos and cyclooxygenase-2 (COX2), modulate synthesis of PGE2 in the amnion during pregnancy and labor

The Expression Patterns of c-Fos and c-Jun Induced by Different Frequencies of Electroacupuncture in the Brain

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15 Customer Q&As for Anti-C-FOS Monoclonal Antibody

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mucosa of stomach using anti-C-FOS Monoclonal antibody M00297-3. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-25

Question

Here is the WB image, lot number and protocol we used for mucosa of stomach using anti-C-FOS Monoclonal antibody M00297-3. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-03-25

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-03-25

Question

We purchased anti-C-FOS Monoclonal antibody for WB on mucosa of stomach in a previous experiment. I am using mouse, and We intend to use the antibody for IF next. My question regards examining mucosa of stomach as well as leukemic t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2019-03-01

Answer

I looked at the website and datasheets of our anti-C-FOS Monoclonal antibody and it seems that M00297-3 has been tested on mouse in both WB and IF. Thus M00297-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-03-01

Question

Does M00297-3 anti-C-FOS Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-06-15

Answer

It shows on the product datasheet, M00297-3 anti-C-FOS Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-06-15

Question

I see that the anti-C-FOS Monoclonal antibody M00297-3 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-03-28

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-03-28

Question

Is this M00297-3 anti-C-FOS Monoclonal antibody reactive to the isotypes of FOS?

Verified Customer

Verified customer

Asked: 2018-02-28

Answer

The immunogen of M00297-3 anti-C-FOS Monoclonal antibody is Full length recombinant human protein expressed in and purified from E. coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-02-28

Question

I was wanting to use your anti-C-FOS Monoclonal antibody for WB for mouse mucosa of stomach on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse mucosa of stomach identification?

Verified Customer

Verified customer

Asked: 2017-10-06

Answer

You can see on the product datasheet, M00297-3 anti-C-FOS Monoclonal antibody has been validated for IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse mucosa of stomach in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-10-06

Question

We are currently using anti-C-FOS Monoclonal antibody M00297-3 for rat tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2017-09-11

Answer

The anti-C-FOS Monoclonal antibody (M00297-3) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-09-11

Question

Is a blocking peptide available for product anti-C-FOS Monoclonal antibody (M00297-3)?

Verified Customer

Verified customer

Asked: 2017-09-01

Answer

We do provide the blocking peptide for product anti-C-FOS Monoclonal antibody (M00297-3). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-09-01

Question

My team were content with the WB result of your anti-C-FOS Monoclonal antibody. However we have observed positive staining in mucosa of stomach nucleus. endoplasmic reticulum. cytoplasm, using this antibody. Is that expected? Could you tell me where is FOS supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-08-21

Answer

According to literature, mucosa of stomach does express FOS. Generally FOS expresses in nucleus. endoplasmic reticulum. cytoplasm,. Regarding which tissues have FOS expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-08-21

Question

I am interested in to test anti-C-FOS Monoclonal antibody M00297-3 on mouse mucosa of stomach for research purposes, then I may be interested in using anti-C-FOS Monoclonal antibody M00297-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-06-29

Answer

The products we sell, including anti-C-FOS Monoclonal antibody M00297-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-06-29

Question

Would anti-C-FOS Monoclonal antibody M00297-3 work for WB with mucosa of stomach?

J. Patel

Verified customer

Asked: 2017-05-29

Answer

According to the expression profile of mucosa of stomach, FOS is highly expressed in mucosa of stomach. So, it is likely that anti-C-FOS Monoclonal antibody M00297-3 will work for WB with mucosa of stomach.

Boster Scientific Support

Answered: 2017-05-29

Question

We have observed staining in human leukemic t-cell. Are there any suggestions? Is anti-C-FOS Monoclonal antibody supposed to stain leukemic t-cell positively?

Z. Yang

Verified customer

Asked: 2015-07-28

Answer

According to literature leukemic t-cell does express FOS. According to Uniprot.org, FOS is expressed in mucosa of stomach, colon, pancreas, leukemic t-cell, among other tissues. Regarding which tissues have FOS expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2015-07-28

Question

Can you help my question with product M00297-3, anti-C-FOS Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

C. Li

Verified customer

Asked: 2015-03-31

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00297-3 anti-C-FOS Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-03-31

Question

Do you have a BSA free version of anti-C-FOS Monoclonal antibody M00297-3 available?

J. Jha

Verified customer

Asked: 2013-03-27

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-C-FOS Monoclonal antibody M00297-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-03-27

$299

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Ships directly to USA and Canada. For other regions, visit our distributors page

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M00297-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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