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Product Info Summary
| SKU: | M02038 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-c-Jun Rabbit Monoclonal Antibody
SKU/Catalog Number
M02038
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-c-Jun Rabbit Monoclonal Antibody catalog # M02038. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-c-Jun Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02038)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CAO-10
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human c-Jun
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02038 is reactive to JUN in Human, Mouse, Rat
Applications
M02038 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
43 kDa
Calculated molecular weight
35.676kDa
Background of c-jun
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of c-Jun using anti-c-Jun antibody (M02038).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Jun antigen affinity purified monoclonal antibody (Catalog # M02038) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Jun at approximately 43 kDa. The expected band size for c-Jun is at 36 kDa.
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Figure 2. IF analysis of c-Jun using anti-c-Jun antibody (M02038).
c-Jun was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 with rabbit anti-c-Jun Antibody (M02038) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat cerebellum, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human squamous carcinoma, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human breast cancer, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle - gastrocnemius , using the Antibody at 1:500 dilution.
Protein Target Info & Infographic
Gene/Protein Information For JUN (Source: Uniprot.org, NCBI)
Gene Name
JUN
Full Name
Transcription factor AP-1
Weight
35.676kDa
Superfamily
bZIP family
Alternative Names
Activator protein 1; AP1; AP-1; cJun; c-Jun; enhancer-binding protein AP1; Jun activation domain binding protein; jun oncogene; jun proto-oncogene; JUN; Proto-oncogene c-Jun; transcription factor AP-1; V-jun avian sarcoma virus 17 oncogene homologp39; v-jun sarcoma virus 17 oncogene homolog (avian); v-jun sarcoma virus 17 oncogene homolog JUN AP-1, AP1, c-Jun, cJUN, p39 Jun proto-oncogene, AP-1 transcription factor subunit transcription factor AP-1|Jun activation domain binding protein|activator protein 1|enhancer-binding protein AP1|jun oncogene|proto-oncogene c-Jun|proto-oncogene cJun|v-jun avian sarcoma virus 17 oncogene homolog|v-jun sarcoma virus 17 oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on JUN, check out the JUN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for JUN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-c-Jun Rabbit Monoclonal Antibody (M02038)
Hello CJ!
M02038 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Hirsutanol A, a novel sesquiterpene compound from fungusChondrostereumsp., induces apoptosis and inhibits tumor growth through mitochondrial-independent ROS production: Hirsutanol A inhibits tumor growth through ROS production
Vasopressin decreases neuronal apoptosis during cardiopulmonary resuscitation
Zhang C, Chang C, Li D, Zhang F, Xu C. Cell Mol Biol Lett. 2017 Sep 16;22:21. doi: 10.1186/s11658-017-0051-3. eCollection 2017. The novel protein C3orf43 accelerates hepatocyte proliferation
Yang Y, Qin J, Lan L, Li N, Wang C, He P, Liu F, Ni H, Wang Y. Cancer Biol Ther. 2014 Jan;15(1):99-107. Doi: 10.4161/Cbt.26718. Epub 2013 Oct 22. M-Csf Cooperating With Nf??b Induces Macrophage Transformation From M1 To M2 By Upregulating C-Jun.
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-c-Jun Rabbit Monoclonal Antibody
Question
Here is the WB image, lot number and protocol we used for amniotic fluid using anti-c-Jun Rabbit Monoclonal antibody M02038. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-01
Question
Is a blocking peptide available for product anti-c-Jun Rabbit Monoclonal antibody (M02038)?
S. Krishna
Verified customer
Asked: 2016-02-17
Answer
We do provide the blocking peptide for product anti-c-Jun Rabbit Monoclonal antibody (M02038). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-02-17
Question
I have a question about product M02038, anti-c-Jun Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Moore
Verified customer
Asked: 2013-12-16
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02038 anti-c-Jun Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-12-16
