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Product Info Summary
| SKU: | M01880 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-c-Rel Monoclonal Antibody
SKU/Catalog Number
M01880
BM5403 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-c-Rel Monoclonal Antibody catalog # M01880. Tested in WB, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-c-Rel Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01880)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADDH-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human c-Rel c-Rel contains an amino-terminal DNA-binding domain referred to as the REL homology domain (REH) and carboxy-terminal transactivation domains. The c-Rel protein is typically inhibited in unstimulated cells by I Kappa B Alpha and I Kappa B Beta . c-Rel expression is highest in hematopoietic cells with extensive research studies demonstrating its role in immune cell function and pathogenesis of disease.
Reactive Species
M01880 is reactive to REL in Human
Observed Molecular Weight
78 kDa
Calculated molecular weight
68.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01880 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:20
Positive Control
WB: human Daudi whole cell, human Caco-2 whole cell, human A549 whole cell, human RT4 whole cell, human K562 whole cell, human Jurkat whole cell
Validation Images & Assay Conditions
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Western blot analysis of c-Rel/REL using anti-c-Rel/REL antibody (M01880).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Rel/REL antigen affinity purified monoclonal antibody (M01880) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Rel/REL at approximately 78 kDa. The expected band size for c-Rel/REL is at 69 kDa.
Specific Publications For Anti-c-Rel Monoclonal Antibody (M01880)
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1 Customer Q&As for Anti-c-Rel Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M01880?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of c-Rel expression in Daudi cell lysate.
Boster Scientific Support
Answered: 2020-01-09
