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- Table of Contents
REL antibody (M01880) performs well in IP, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of c-Rel/REL using anti-c-Rel/REL antibody (M01880).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Rel/REL antigen affinity purified monoclonal antibody (M01880) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Rel/REL at approximately 78 kDa. The expected band size for c-Rel/REL is at 69 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Rel/REL antigen affinity purified monoclonal antibody (M01880) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Rel/REL at approximately 78 kDa. The expected band size for c-Rel/REL is at 69 kDa.
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Anti-c-Rel Monoclonal Antibody
REL antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IP, WB |
Tested Applications
REL antibody was positively detected by WB in:
human Daudi whole cell, human Caco-2 whole cell, human A549 whole cell, human RT4 whole cell, human K562 whole cell, human Jurkat whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IP 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-c-Rel Monoclonal Antibody
View all REL Antibodies
View all REL Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADDH-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human c-Rel c-Rel contains an amino-terminal DNA-binding domain referred to as the REL homology domain (REH) and carboxy-terminal transactivation domains. The c-Rel protein is typically inhibited in unstimulated cells by I Kappa B Alpha and I Kappa B Beta . c-Rel expression is highest in hematopoietic cells with extensive research studies demonstrating its role in immune cell function and pathogenesis of disease.
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
78 kDa
Calculated Molecular Weight
68.5 kDa
Cite This Product
Anti-c-Rel Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01880)
Protocols
Western Blot protocol for REL antibody
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M01880
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 19, 2026