Anti-CA2 Antibody Picoband™ (monoclonal, 10E11)

Boster Bio Anti-CA2 Antibody Picoband™ (monoclonal, 10E11) catalog # M00143. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M00143
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-CA2 Antibody Picoband™ (monoclonal, 10E11)

See all Carbonic Anhydrase II/CA2 products

SKU/Catalog Number

M00143

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CA2 Antibody Picoband™ (monoclonal, 10E11) catalog # M00143. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CA2 Antibody Picoband™ (monoclonal, 10E11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00143)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

10E11

Isotype

IgG2b

Immunogen

E.coli-derived human CA2 recombinant protein (Position: S2-K260). Human CA2 shares 81.1% and 80.7% amino acid (aa) sequence identity with mouse and rat CA2, respectively.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M00143 is reactive to CA2 in Human, Mouse, Rat

Applications

M00143 is guaranteed for Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For CA2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P00918

Gene ID

760

Gene Name

CA2

Full Name

Carbonic anhydrase 2

Weight

29.246kDa

Superfamily

alpha-carbonic anhydrase family

Alternative Names

CA2; CAII; CA-IIEC 4.2.1.1; Car2; Carbonate dehydratase II; carbonic anhydrase 2; carbonic anhydrase B; Carbonic anhydrase C; Carbonic Anhydrase II; carbonic anhydrase IICAC; carbonic dehydratase CA2 CA-II, CAC, CAII, Car2, HEL-76, HEL-S-282 carbonic anhydrase 2 carbonic anhydrase 2|carbonate dehydratase II|carbonic anhydrase B|carbonic anhydrase C|carbonic anhydrase II|carbonic dehydratase|epididymis luminal protein 76|epididymis secretory protein Li 282

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CA2, check out the CA2 Infographic

CA2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CA2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00143 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Calcium-and integrin-binding protein-1 and calcineurin are upregulated in the right atrial myocardium of patients with atrial fibrillation

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6 Customer Q&As for Anti-CA2 Antibody Picoband™ (monoclonal, 10E11)

Question

Would anti-CA2 antibody (monoclonal, 10E11) M00143 work on zebrafish IHC-P with urinary bladder?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

Our lab technicians have not validated anti-CA2 antibody (monoclonal, 10E11) M00143 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-CA2 antibody (monoclonal, 10E11) M00143 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish urinary bladder in IHC-P, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-19

Question

We want to test anti-CA2 antibody (monoclonal, 10E11) M00143 on rat urinary bladder for research purposes, then I may be interested in using anti-CA2 antibody (monoclonal, 10E11) M00143 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

C. Jones

Verified customer

Asked: 2019-10-25

Answer

The products we sell, including anti-CA2 antibody (monoclonal, 10E11) M00143, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-25

Question

See attached the WB image, lot number and protocol we used for urinary bladder using anti-CA2 antibody (monoclonal, 10E11) M00143. Please let me know if you require anything else.

E. Li

Verified customer

Asked: 2019-10-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-10

Question

Would M00143 anti-CA2 antibody (monoclonal, 10E11) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

J. Jackson

Verified customer

Asked: 2019-08-16

Answer

It shows on the product datasheet, M00143 anti-CA2 antibody (monoclonal, 10E11) as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-16

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for urinary bladder using anti-CA2 antibody (monoclonal, 10E11) M00143. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-07-09

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-09

Question

Is this M00143 anti-CA2 antibody (monoclonal, 10E11) reactive to the isotypes of CA2?

D. Krishna

Verified customer

Asked: 2015-06-05

Answer

The immunogen of M00143 anti-CA2 antibody (monoclonal, 10E11) is E.coli-derived human CA2 recombinant protein (Position: S2-K260). Human CA2 shares 81.1% and 80.7% amino acid (aa) sequence identity with mouse and rat CA2, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2015-06-05

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