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Product Info Summary
| SKU: | A01406-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-CACNA1H Antibody
SKU/Catalog Number
A01406-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CACNA1H Antibody (Catalog # A01406-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CACNA1H antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-CACNA1H Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01406-1)
Host
Rabbit
Contents
CACNA1H antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CACNA1H antibody was raised against a 16 amino acid peptide near the center of human CACNA1H. The immunogen is located within amino acids 1010 - 1060 of CACNA1H.
Cross-reactivity
CACNA1H antibody is human, mouse and rat reactive. Multiple isoforms of CACNA1H are known to exist.
Reactive Species
A01406-1 is reactive to CACNA1H in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
259.2 kDa
Background of CACNA1H
The calcium channel protein CACNA1H is a T-type member of the alpha-1 subunit family that is part of the voltage-dependent calcium channel complex which mediates the influx of calcium ions into the cell upon membrane polarization (1,2). CACNA1H is a subunit of Cav3.2 T-type calcium channel that is involved in neurological disorders such as epilepsy and pain (2,3). CACNA1H associates with the caveolin protein Cav-3 and it is thought that Cav-3 regulates the Protein Kinase A (PKA) modulation of CACNA1H-containing Cav3.2 T-type calcium channels (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01406-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CACNA1H antibody can be used for detection of CACNA1H by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CACNA1H in 293 cell lysate with CACNA1H antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of CACNA1H in mouse brain tissue with CACNA1H antibody at 5 μg/ml.
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Immunofluorescence of CACNA1H in mouse brain tissue with CACNA1H antibody at 20 μg/ml.
Specific Publications For Anti-CACNA1H Antibody (A01406-1)
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