Anti-CAF1B CHAF1B Antibody

CHAF1B antibody

Boster Bio Anti-CAF1B CHAF1B Antibody (Catalog# A30479). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A30479
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC-P, ICC, WB

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Product Name

Anti-CAF1B CHAF1B Antibody

View all CHAF1B Antibodies

SKU/Catalog Number

A30479

Size

100ul

Form

Liquid

Description

Boster Bio Anti-CAF1B CHAF1B Antibody (Catalog# A30479). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CAF1B CHAF1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30479)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human CAF1B. Immunogen sequence location: 71-120

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A30479 is reactive to CHAF1B in Human, Mouse, Rat

Applications

A30479 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

61.493kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:20000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For CHAF1B (Source: Uniprot.org, NCBI)

Gene Name

CHAF1B

Full Name

Chromatin assembly factor 1 subunit B

Weight

61.493kDa

Superfamily

WD repeat HIR1 family

Alternative Names

CAF1; CAF-1; CAF1ACAF-1 subunit B; CAF1P60CAF-I 60 kDa subunit; CAF-IP60; chromatin assembly factor 1 subunit B; chromatin assembly factor 1, subunit B (p60); Chromatin assembly factor I p60 subunit; human chromatin assembly factor-I p60 subunit, 10; MPHOSPH7CAF-I p60; MPP7M-phase phosphoprotein 7 CHAF1B CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7 chromatin assembly factor 1 subunit B chromatin assembly factor 1 subunit B|CAF-1 subunit B|CAF-I 60 kDa subunit|CAF-I p60|M-phase phosphoprotein 7|chromatin assembly factor I p60 subunit|human chromatin assembly factor-I p60 subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CHAF1B, check out the CHAF1B Infographic

CHAF1B infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CHAF1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-CAF1B CHAF1B Antibody

Question

Would anti-CAF1B antibody A30479 work for WB with cervix carcinoma erythroleukemia?

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

According to the expression profile of cervix carcinoma erythroleukemia, CHAF1B is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-CAF1B antibody A30479 will work for WB with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2020-03-31

Question

Would A30479 anti-CAF1B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

S. Collins

Verified customer

Asked: 2020-03-10

Answer

As indicated on the product datasheet, A30479 anti-CAF1B antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-10

Question

Do you have a BSA free version of anti-CAF1B antibody A30479 available?

Verified Customer

Verified customer

Asked: 2019-11-11

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CAF1B antibody A30479 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-11-11

Question

I see that the anti-CAF1B antibody A30479 works with WB, what is the protocol used to produce the result images on the product page?

M. Jones

Verified customer

Asked: 2019-07-10

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-10

Question

Will anti-CAF1B antibody A30479 work on pig WB with cervix carcinoma erythroleukemia?

B. Li

Verified customer

Asked: 2018-10-24

Answer

Our lab technicians have not validated anti-CAF1B antibody A30479 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CAF1B antibody A30479 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-10-24

Question

We are currently using anti-CAF1B antibody A30479 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?

S. Edwards

Verified customer

Asked: 2018-03-02

Answer

The anti-CAF1B antibody (A30479) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-03-02

Order DetailsPrice
A30479

100 μL

$335

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A30479
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00

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