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Product Info Summary
| SKU: | A30479 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CAF1B CHAF1B Antibody
SKU/Catalog Number
A30479
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CAF1B CHAF1B Antibody (Catalog# A30479). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CAF1B CHAF1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30479)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CAF1B. Immunogen sequence location: 71-120
Reactive Species
A30479 is reactive to CHAF1B in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
61.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30479 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western blot analysis of lysates from Jurkat cells, using CAF1B Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from RAW264.7cells using CAF1B antibody.
Specific Publications For Anti-CAF1B CHAF1B Antibody (A30479)
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Customer Q&As
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6 Customer Q&As for Anti-CAF1B CHAF1B Antibody
Question
Would anti-CAF1B antibody A30479 work for WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
According to the expression profile of cervix carcinoma erythroleukemia, CHAF1B is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-CAF1B antibody A30479 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2020-03-31
Question
Would A30479 anti-CAF1B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Collins
Verified customer
Asked: 2020-03-10
Answer
As indicated on the product datasheet, A30479 anti-CAF1B antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-10
Question
Do you have a BSA free version of anti-CAF1B antibody A30479 available?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CAF1B antibody A30479 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-11
Question
I see that the anti-CAF1B antibody A30479 works with WB, what is the protocol used to produce the result images on the product page?
M. Jones
Verified customer
Asked: 2019-07-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-10
Question
Will anti-CAF1B antibody A30479 work on pig WB with cervix carcinoma erythroleukemia?
B. Li
Verified customer
Asked: 2018-10-24
Answer
Our lab technicians have not validated anti-CAF1B antibody A30479 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CAF1B antibody A30479 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-24
Question
We are currently using anti-CAF1B antibody A30479 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
S. Edwards
Verified customer
Asked: 2018-03-02
Answer
The anti-CAF1B antibody (A30479) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-02
