Anti-Calcineurin Alpha Ppp3r1 Antibody (Monoclonal, CN-A1)

Product Name

Anti-Calcineurin Alpha Ppp3r1 Antibody (Monoclonal, CN-A1)

View all Calcineurin B Antibodies

SKU/Catalog Number

MA1008

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Calcineurin Alpha Ppp3r1 Antibody (Monoclonal, CN-A1) catalog # MA1008. Tested in IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rat.

Storage & Handling

At -20˚C for one year and at 4˚C for one month from date of receipt. Avoid repeated freezing and thawing.

Cite This Product

Anti-Calcineurin Alpha Ppp3r1 Antibody (Monoclonal, CN-A1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1008)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

CN-A1

Isotype

Mouse IgG1

Immunogen

Bovine brain calcineurin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1008 is reactive to PPP3R1 in Bovine, Human, Mouse, Rat

Applications

MA1008 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

61 kDa

Calculated molecular weight

19.3kDa

Background of Calcineurin B

Calcineurin, the Ca (2+)/calmodulin-regulated protein phosphatase, first detected in skeletal muscle and brain, has been found in all cells from yeast to mammals. Calcineurin A alpha (PPP3CA),is located on human chromosomes 4, Chromosomal mapping of the human genes for the calmodulin-dependent protein phosphatase (calcineurin) catalytic subunit. Calcineurin regulates bone formation by the osteoblast.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 1 ml of PBS buffer will yield a concentration of 100 ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, bovine, mouse, rat, By Heat
Western blot, 0.25-0.5μg/ml, Human, bovine, mouse, rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Calcineurin Alpha/PPP3R1 using anti-Calcineurin Alpha/PPP3R1 antibody (MA1008).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Calcineurin Alpha/PPP3R1 antigen affinity purified monoclonal antibody (Catalog # MA1008) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Calcineurin Alpha/PPP3R1 at approximately 61 kDa. The expected band size for Calcineurin Alpha/PPP3R1 is at 19 kDa.

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Figure 2. IHC analysis of Calcineurin Alpha/PPP3R1 using anti-Calcineurin Alpha/PPP3R1 antibody (MA1008).
Calcineurin Alpha/PPP3R1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-Calcineurin Alpha/PPP3R1 Antibody (MA1008) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

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Figure 3. IHC analysis of Calcineurin Alpha/PPP3R1 using anti-Calcineurin Alpha/PPP3R1 antibody (MA1008).
Calcineurin Alpha/PPP3R1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-Calcineurin Alpha/PPP3R1 Antibody (MA1008) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

Gene/Protein Information For PPP3R1 (Source: Uniprot.org, NCBI)

Alternative Names

Calcineurin B; calcineurin subunit B type 1; CALNB1; CNA2; Cnb1; MCIP1; PP2B beta 1; PPP3R1; Protein phosphatase 2B regulatory subunit 1; protein phosphatase 2B regulatory subunit B alpha; protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform(calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform(calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform; Protein phosphatase 3 regulatory subunit B alpha isoform 1; protein phosphatase 3, regulatory subunit B, alpha; type I (19kDa) PPP3R1 CALNB1, CNB, CNB1 protein phosphatase 3 regulatory subunit B, alpha calcineurin subunit B type 1|calcineurin B, type I (19kDa)|protein phosphatase 2B regulatory subunit 1|protein phosphatase 2B regulatory subunit B alpha

For more info on PPP3R1, check out the PPP3R1 Infographic

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In this infographic, you will see the following information for PPP3R1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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