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- Table of Contents
Calca antibody (PB9937) performs well in IHC and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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IHC analysis of Survivin/Calcitonin/CALCA using anti-Survivin/Calcitonin/CALCA antibody (PB9937).
Survivin/Calcitonin/CALCA was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Survivin/Calcitonin/CALCA Antibody (PB9937) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Survivin/Calcitonin/CALCA was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Survivin/Calcitonin/CALCA Antibody (PB9937) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Calcitonin/CALCA Antibody
Calca antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | IHC |
Tested Applications
Calca antibody was positively detected by IHC in:
mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
Mouse
Tested
Recommend
Rat
Tested
Recommend
Bovine
Predicted
Predicted
Dog
Predicted
Predicted
Product Information
Product Name
Anti-Calcitonin/CALCA Antibody
View all CALCA Antibodies
View all CALCA Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Calcitonin, different from the related human sequence by four amino acids, and identical to the related rat sequence.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Calculated Molecular Weight
14.1 kDa
Background of Calca
Calcitonin, also known as CALCA, is a peptide hormone synthesized by the parafollicular cells of the thyroid. It is mapped to 11p15.2. Calcitonin belongs to the calcitonin-like protein family. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.
Cite This Product
Anti-Calcitonin/CALCA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9937)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
100%
High Homology
Cow
97%
High Homology
Dog
97%
High Homology
Human
89%
Moderate Homology
Rabbit
86%
Moderate Homology
Zebrafish
76%
Moderate Homology
Monkey
24%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for Calca antibody
Publications
Application:
Species:
Effect of chronic hypoxia and hypercapnia on learning and memory function in mice and the expression of NT and CGRP in brain
Published: 2018-12-20
Journal: European Journal of Inflammation
Application: IHC
Impact Factor: 0.94
Species: Mouse
The integration of NSC-derived and host neural networks after rat spinal cord transection
Published: 2013-01-26
Journal: BIOMATERIALS
Application: IF
Impact Factor: 7.60
Species: Rat
PMID: 23360782
Analgesic effect of diprospan in rats with trigeminal neuralgia
Published: 2011-06-14
Journal: Journal of Huazhong University of Science and Technology-Medical Sciences
Application: IHC
Impact Factor: 0.41
Species: Rat
PMID: 21671185
Effects of Calcitonin Gene-Related Peptide on the Expression and Activity of Nitric Oxide Synthase During Mandibular Bone Healing in Rabbits: An Experimental Study
Published: 2009-01-09
Journal: JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
Application: IHC
Impact Factor: 1.24
Species: Rabbit
PMID: 19138599
The colocalization of CGRP receptor and AMPA receptor in the spinal dorsal horn neuron of rat: A morphological and electrophysiological study
Published: 2007-01-18
Journal: NEUROSCIENCE LETTERS
Application: IF
Impact Factor: 2.09
Species: Rat
PMID: 17287081
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Product Promise
We promise Anti-Calcitonin/CALCA Antibody performs excellently in relevant applications for IHC. If it doesn't perform as promised, we will refund the product. Learn more
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PB9937
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026