Product Info Summary
SKU: | A03492-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-Calpain 2 CAPN2 Antibody
SKU/Catalog Number
A03492-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Calpain 2 CAPN2 Antibody catalog # A03492-1. Tested in WB,ICC,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calpain 2 CAPN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03492-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MT-ND5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03492-1 is reactive to CAPN2 in Human, Mouse, Rat
Applications
A03492-1 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
79.995kDa
Background of Calpain 2
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Anderson S., Nature 290:457-465(1981).
Horai S., Proc. Natl. Acad. Sci. U.S.A. 92:532-536(1995).
Maca-Meyer N., BMC Genet. 2:13-13(2001).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of CAPN2 using Anti-Calpain 2 CAPN2 Antibody (A03492-1).
ICC staining Calpain 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Calpain 2 CAPN2 Antibody A03492-1
Western blot analysis of Calpain 2 on Hela cell (1) and rat skin tissue (2) lysate using anti-Calpain 2 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For CAPN2 (Source: Uniprot.org, NCBI)
Gene Name
CAPN2
Full Name
Calpain-2 catalytic subunit
Weight
79.995kDa
Superfamily
peptidase C2 family
Alternative Names
Calcium-activated neutral proteinase 2; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; Calpain large polypeptide L2; Calpain M-type; calpain, large polypeptide L2; calpain-2 catalytic subunit; Calpain-2 large subunit; CANP 2; CANPL2CANP2; CANPml; EC 3.4.22; EC 3.4.22.53; FLJ39928; M-calpain; mCANP; millimolar-calpain CAPN2 CANP2, CANPL2, CANPml, mCANP calpain 2 calpain-2 catalytic subunit|CANP 2|M-calpain|calcium-activated neutral proteinase 2|calpain 2, (m/II) large subunit|calpain 2, large [catalytic] subunit|calpain 2, large subunit|calpain M-type|calpain, large polypeptide L2|millimolar-calpain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CAPN2, check out the CAPN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CAPN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Calpain 2 CAPN2 Antibody (A03492-1)
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No publications found for A03492-1
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