Anti-CaM Kinase IV CAMK4 Antibody

Product Name

Anti-CaM Kinase IV CAMK4 Antibody

View all CaMKIV Antibodies

SKU/Catalog Number

A01905

Size

100uL

Form

Lyophilized

Description

Boster Bio Anti-CaM Kinase IV CAMK4 Antibody (Catalog # A01905). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-CaM Kinase IV CAMK4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01905)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

This antiserum was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 300-325 of Human CaM Kinase IV protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01905 is reactive to CAMK4 in Human, Mouse, Rat

Applications

A01905 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

51.926kDa

Background of CaMKIV

CaM Kinase IV (also known as CAM kinase-GR and CaMK IV) is a calcium/ calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. This kinase may be involved in the transcriptional regulation of microtubule dynamics. In vitro, CaMK IV phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. CaMK IV may also be involved in spermatogenesis and may play a role in the consolidation/ retention of hippocampus-dependent long-term memory. CaMK IV must be phosphorylated to be maximally active and is phosphorylated by CAMKK1 or CAMKK2. In addition autophosphorylation of the N-terminus is required for full activation. Autophosphorylation of Ser-336 allows the kinase to switch to a Ca(2+)/calmodulin-independent state. Most likely the kinase is inactivated by the serine/ threonine protein phosphatase 2A. CaMK IV is a monomer that is located within the cytoplasm and nucleus and substantial localization occurs in certain neuronal nuclei. In spermatids CaMK IV is associated with chromatin and the nuclear matrix. CaMK IV is also specifically expressed in epithelial ovarian cancer tissue.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:25,000
IHC: 1:500
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

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Western blot analysis of CaM Kinase IV (arrowhead) expression in rat brain extract (lane 1), Jurkat cell lysates (lane 2), specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 3 and 4 respectively) using rabbit anti-CaM Kinase IV polyclonal antibody (Catalog # A01905) at 1:1000.

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CaM Kinase IV was detected in paraffin-embedded sections of human brain cortex tissues using rabbit anti-CaM Kinase IV polyclonal antibody (Catalog # A01905) at 1:500. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Gene/Protein Information For CAMK4 (Source: Uniprot.org, NCBI)

Alternative Names

brain Ca(2+)-calmodulin-dependent protein kinase type IV; brain Ca++-calmodulin-dependent protein kinase type IV; calcium/calmodulin-dependent protein kinase IV; calcium/calmodulin-dependent protein kinase type IV catalytic chain; calcium/calmodulin-dependent protein kinase type IV; CAM kinase- GR; CAM kinase IV; CaM kinase-GR; CaMK IV; caMK; CaMK-GR; EC 2.7.11; EC 2.7.11.17; IV; MGC36771 CAMK4 CaMK IV, CaMK-GR, CaMKIV, caMK calcium/calmodulin dependent protein kinase IV calcium/calmodulin-dependent protein kinase type IV|CAM kinase IV|CAM kinase- GR|brain Ca(2+)-calmodulin-dependent protein kinase type IV|brain Ca++-calmodulin-dependent protein kinase type IV|calcium/calmodulin-dependent protein kinase type IV catalytic chain

For more info on CAMK4, check out the CAMK4 Infographic

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In this infographic, you will see the following information for CAMK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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