Product Info Summary
| SKU: | A30525 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CAMK5 CAMKV Antibody
SKU/Catalog Number
A30525
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CAMK5 CAMKV Antibody (Catalog# A30525). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CAMK5 CAMKV Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30525)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CAMK5. Immunogen sequence location: 211-260
Reactive Species
A30525 is reactive to CAMKV in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
54.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30525 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of various cells using CaMKV Polyclonal Antibody
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Immunofluorescence analysis of HeLa cells, using CAMK5 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CAMK5 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HepG2 and HUVEC cells, using CAMK5 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CAMK5 CAMKV Antibody (A30525)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-CAMK5 CAMKV Antibody
Question
See below the WB image, lot number and protocol we used for brain lung using anti-CAMK5 antibody A30525. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-30
Question
Is this A30525 anti-CAMK5 antibody reactive to the isotypes of CAMKV?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
The immunogen of A30525 anti-CAMK5 antibody is A peptide derived from human CAMK5. Immunogen sequence location: 211-260. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain lung using anti-CAMK5 antibody A30525. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-08
Question
Would anti-CAMK5 antibody A30525 work for WB with brain lung?
Verified Customer
Verified customer
Asked: 2019-05-07
Answer
According to the expression profile of brain lung, CAMKV is highly expressed in brain lung. So, it is likely that anti-CAMK5 antibody A30525 will work for WB with brain lung.
Boster Scientific Support
Answered: 2019-05-07
Question
We are currently using anti-CAMK5 antibody A30525 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-02
Answer
The anti-CAMK5 antibody (A30525) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-02
Question
Would anti-CAMK5 antibody A30525 work on feline IF with frontal cortex?
C. Rodriguez
Verified customer
Asked: 2016-12-02
Answer
Our lab technicians have not validated anti-CAMK5 antibody A30525 on feline. You can run a BLAST between feline and the immunogen sequence of anti-CAMK5 antibody A30525 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline frontal cortex in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-12-02

