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- Table of Contents
CNR1 antibody (A01291-1) performs well in ELISA, Flow Cytometry, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Flow Cytometry analysis of Hela cells using anti-CNR1 antibody (A01291-1).
Overlay histogram showing Hela cells stained with A01291-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CNR1 Antibody (A01291-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Hela cells stained with A01291-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CNR1 Antibody (A01291-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Cannabinoid Receptor I/CNR1 Antibody Picoband®
CNR1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, ICC, WB |
Tested Applications
CNR1 antibody was positively detected by WB in:
human 22RV1 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CNR1 antibody was positively detected by IHC in:
mouse small intestine tissue, rat small intestine tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CNR1 antibody was positively detected by FC in:
Hela cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Immunocytochemistry
0.5-1μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
ICC
IF
ELISA
Human
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Cannabinoid Receptor I/CNR1 Antibody Picoband®
View all CNR1 Antibodies
View all CNR1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Cannabinoid Receptor I recombinant protein (Position: M1-Q75).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated Molecular Weight
52.9 kDa
Background of CNR1
The cannabinoid receptor type 1, often abbreviated as CB1, is a G protein-coupled cannabinoid receptor located primarily in the central and peripheral nervous system. This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene.
Cite This Product
Anti-Cannabinoid Receptor I/CNR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01291-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Mouse
96%
High Homology
Rat
96%
High Homology
Sheep
96%
High Homology
Cow
95%
High Homology
Zebrafish
51%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CNR1 antibody
Immunohistochemistry (IHC) protocol for CNR1 antibody
Flow Cytometry (FC) protocol for CNR1 antibody
Publications
Application:
Species:
In Vivo and In Vitro Crosstalk Among CBD, Aβ, and Endocannabinoid System Enzymes and Receptors
Published: 2025-05-09
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: FC
Impact Factor: 4.70
Species: Rat
PMID: 40350019
The Roles of Anandamide, Fatty Acid Amide Hydrolase, and Leukemia Inhibitory Factor on the Endometrium during the Implantation Window
Published: 2017-10-16
Journal: Frontiers in Endocrinology
Application: WB
Impact Factor: 3.68
Species: Human
PMID: 29085337
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Product Promise
We promise Anti-Cannabinoid Receptor I/CNR1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A01291-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026