Anti-Carboxypeptidase A/CPA1 Antibody Picoband™

Product Name

Anti-Carboxypeptidase A/CPA1 Antibody Picoband™

View all Carboxypeptidase A1/CPA1 Antibodies

SKU/Catalog Number

A05985-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Carboxypeptidase A/CPA1 Antibody Picoband™ catalog # A05985-1. Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Carboxypeptidase A/CPA1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05985-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Carboxypeptidase A, which shares 84.8% and 87% amino acid (aa) sequence identity with mouse and rat Carboxypeptidase A, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05985-1 is reactive to CPA1 in Mouse, Rat

Applications

A05985-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

47 kDa

Calculated molecular weight

47.14kDa

Background of Carboxypeptidase A1/CPA1

Carboxypeptidase A1 is an enzyme that in humans is encoded by the CPA1 gene. This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. This enzyme is produced in the pancreas and preferentially cleaves C-terminal branched-chain and aromatic amino acids from dietary proteins. This gene and several family members are present in a gene cluster on chromosome 7. Mutations in this gene may be linked to chronic pancreatitis, while elevated protein levels may be associated with pancreatic cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Carboxypeptidase A/CPA1 using anti-Carboxypeptidase A/CPA1 antibody (A05985-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat pancreas tissue lysates,
Lane 2: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carboxypeptidase A/CPA1 antigen affinity purified polyclonal antibody (Catalog # A05985-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Carboxypeptidase A/CPA1 at approximately 47 kDa. The expected band size for Carboxypeptidase A/CPA1 is at 47 kDa.

Gene/Protein Information For CPA1 (Source: Uniprot.org, NCBI)

Alternative Names

carboxypeptidase A1 (pancreatic); Carboxypeptidase A1; CPA; CPA1; EC 3.4.17; EC 3.4.17.1 CPA1 CPA carboxypeptidase A1 carboxypeptidase A1|carboxypeptidase A1 (pancreatic)|pancreatic carboxypeptidase A

For more info on CPA1, check out the CPA1 Infographic

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In this infographic, you will see the following information for CPA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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