Product Info Summary
SKU: | A00169 |
---|---|
Size: | 100μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IP, ICC, WB |
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Product info
Product Name
Anti-Cardif MAVS Antibody
SKU/Catalog Number
A00169
Size
100μg/vial
Form
Liquid
Description
Boster Bio Anti-Cardif MAVS Antibody catalog # A00169. Tested in ICC, IP, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cardif MAVS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00169)
Host
Rabbit
Contents
Each vial contains 0.02% sodium azide.
Clonality
Polyclonal
Immunogen
Recombinant human Cardif (CARD adapter inducing interferon-beta ) (aa 160-450).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00169 is reactive to MAVS in Human
Applications
A00169 is guaranteed for IP, ICC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
56.528kDa
Background of MAVS
Huntingtin (also known as Huntington's disease protein, Htt and HD protein) is the protein product of a disease gene linked to Huntington's disease, a neuro-degenerative disorder characterized by loss of striatal neurons. This may be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product (see partial protein sequence below). The huntingtin gene locus is large, spanning 180 kb and consisting of 67 exons. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. Normal huntingtin protein shows a cytoplasmic localization. This protein is widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. Anti-Huntington antibodies are ideal for researchers interested in Apoptosis, Autophagy, Cytoskeleton, Neurodegeneration, Neuroscience, and Neuronal Cell Markers research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:2, 000, Human
ICC, 1:500, Human
IP, 1:100, Human
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Cardif expression in MW marker (lane 1), HEPG2 whole cell lysates (lane 2), PALA whole cell lysates (lane 3) and HELA whole cell lysates (lane 4). Cardif at 35/37kD, 18kD, 10kD was detected using rabbit anti-Cardif Antigen Affinity purified polyclonal antibody (Catalog # A00169) at 1: 2000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For MAVS (Source: Uniprot.org, NCBI)
Gene Name
MAVS
Full Name
Mitochondrial antiviral-signaling protein
Weight
56.528kDa
Alternative Names
CARD adapter inducing interferon beta; CARD adapter inducing interferon-beta; CARD adaptor inducing IFN-beta; Cardif; DKFZp547C224; DKFZp666M015; FLJ27482; IFN-B promoter stimulator 1; Interferon beta promoter stimulator protein 1; interferon-beta promoter stimulator protein 1; IPS-1; IPS-1FLJ41962; IPS1MGC3260; KIAA1271FLJ35386; MAVS; mitochondrial antiviral signaling protein; mitochondrial antiviral-signaling protein; mitochondrial viral signaling protein; Putative NF-kappa-B-activating protein 031N; virus-induced signaling adapter variant 1b; virus-induced signaling adaptor variant 1a; Viru MAVS CARDIF, IPS-1, IPS1, VISA mitochondrial antiviral signaling protein mitochondrial antiviral-signaling protein|CARD adapter inducing interferon beta|CARD adaptor inducing IFN-beta|IFN-B promoter stimulator 1|interferon beta promoter stimulator protein 1|putative NF-kappa-B-activating protein 031N|virus-induced signaling adaptor|virus-induced-signaling adapter
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAVS, check out the MAVS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAVS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cardif MAVS Antibody (A00169)
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No publications found for A00169
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-Cardif MAVS Antibody
Question
My lab would like to test anti-Cardif antibody A00169 on human brain for research purposes, then I may be interested in using anti-Cardif antibody A00169 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
The products we sell, including anti-Cardif antibody A00169, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-31
Question
Do you have a BSA free version of anti-Cardif antibody A00169 available?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Cardif antibody A00169 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-09
Question
Would A00169 anti-Cardif antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Evans
Verified customer
Asked: 2019-12-23
Answer
You can see on the product datasheet, A00169 anti-Cardif antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-23
Question
See below the WB image, lot number and protocol we used for brain using anti-Cardif antibody A00169. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-27
Question
My question regarding product A00169, anti-Cardif antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00169 anti-Cardif antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-06
Question
Would anti-Cardif antibody A00169 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
According to the expression profile of brain, MAVS is highly expressed in brain. So, it is likely that anti-Cardif antibody A00169 will work for WB with brain.
Boster Scientific Support
Answered: 2019-07-04
Question
We are currently using anti-Cardif antibody A00169 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
The anti-Cardif antibody (A00169) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-31
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Cardif antibody A00169. Let me know if you need anything else.
P. Parker
Verified customer
Asked: 2018-12-07
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-07
Question
Is a blocking peptide available for product anti-Cardif antibody (A00169)?
B. Gonzalez
Verified customer
Asked: 2018-02-07
Answer
We do provide the blocking peptide for product anti-Cardif antibody (A00169). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-02-07
Question
My team were satisfied with the WB result of your anti-Cardif antibody. However we have been able to see positive staining in leukemic t-cell mitochondrion outer membrane using this antibody. Is that expected? Could you tell me where is MAVS supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
Based on literature, leukemic t-cell does express MAVS. Generally MAVS expresses in mitochondrion outer membrane. Regarding which tissues have MAVS expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10574462, 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung fibroblast, Pubmed ID: 12761501
Pericardium, Placenta, and Tongue, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2018-01-29
Question
We need using your anti-Cardif antibody for traf3-dependent irf activation pathway studies. Has this antibody been tested with western blotting on hepg2 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
Thanks for your inquiry. This A00169 anti-Cardif antibody is validated on hepg2 whole cell lysates, hela whole cell lysates. It is guaranteed to work for IP, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-10-09
Question
We have been able to see staining in human platelet. Are there any suggestions? Is anti-Cardif antibody supposed to stain platelet positively?
E. Baker
Verified customer
Asked: 2016-04-25
Answer
According to literature platelet does express MAVS. According to Uniprot.org, MAVS is expressed in caecum, brain, lung fibroblast, pericardium, placenta tongue, cervix carcinoma, platelet, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MAVS expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10574462, 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung fibroblast, Pubmed ID: 12761501
Pericardium, Placenta, and Tongue, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2016-04-25
Question
Is this A00169 anti-Cardif antibody reactive to the isotypes of MAVS?
C. Anderson
Verified customer
Asked: 2015-10-12
Answer
The immunogen of A00169 anti-Cardif antibody is Recombinant human Cardif (CARD adapter inducing interferon-beta ) (aa 160-450). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-10-12
Question
I was wanting to use your anti-Cardif antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
B. Bhatt
Verified customer
Asked: 2014-08-04
Answer
As indicated on the product datasheet, A00169 anti-Cardif antibody has been tested for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-08-04
Question
We ordered your anti-Cardif antibody for WB on pericardium last year. I am using human, and I plan to use the antibody for IP next. I am looking for examining pericardium as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
R. Parker
Verified customer
Asked: 2014-01-07
Answer
I viewed the website and datasheets of our anti-Cardif antibody and it appears that A00169 has been validated on human in both WB and IP. Thus A00169 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-01-07
Question
I see that the anti-Cardif antibody A00169 works with WB, what is the protocol used to produce the result images on the product page?
S. Collins
Verified customer
Asked: 2013-09-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-09-19