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Product Info Summary
| SKU: | A02468-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-CASK Antibody Picoband™
SKU/Catalog Number
A02468-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CASK Antibody Picoband™ catalog # A02468-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CASK Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02468-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CASK recombinant protein (Position: R27-D498).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02468-2 is reactive to CASK in Human, Mouse, Rat
Applications
A02468-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
120 kDa
Calculated molecular weight
105.123kDa
Background of CASK
Peripheral plasma membrane protein CASK is a protein that in humans is encoded by the CASK gene. This gene encodes a calcium/calmodulin-dependent serine protein kinase. The encoded protein is a MAGUK (membrane-associated guanylate kinase) protein family member. These proteins are scaffold proteins and the encoded protein is located at synapses in the brain. Mutations in this gene are associated with FG syndrome 4, intellectual disability and microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked intellectual disability. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CASK using anti-CASK antibody (A02468-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human CACO-2 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse kidney tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASK antigen affinity purified polyclonal antibody (Catalog # A02468-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASK at approximately 120 kDa. The expected band size for CASK is at 120 kDa.
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Figure 2. Flow Cytometry analysis of CACO-2 cells using anti-CASK antibody (A02468-2).
Overlay histogram showing CACO-2 cells stained with A02468-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CASK Antibody (A02468-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CASK (Source: Uniprot.org, NCBI)
Gene Name
CASK
Full Name
Peripheral plasma membrane protein CASK
Weight
105.123kDa
Superfamily
MAGUK family
Alternative Names
calcium/calmodulin-dependent serine protein kinase (MAGUK family); calcium/calmodulin-dependent serine protein kinase membrane-associatedguanylate kinase; Calcium/calmodulin-dependent serine protein kinase; CAMGUK; CMG; EC 2.7.11; EC 2.7.11.1; FGS4CAGH39; FLJ22219; hCASK; LIN2FLJ31914; MICPCH; peripheral plasma membrane protein CASK; Protein lin-2 homolog; TNRC8; trinucleotide repeat containing 8 CASK CAGH39, CAMGUK, CMG, FGS4, LIN2, MICPCH, MRXSNA, TNRC8, hCASK calcium/calmodulin dependent serine protein kinase peripheral plasma membrane protein CASK|calcium/calmodulin-dependent serin protein kinase|calcium/calmodulin-dependent serine protein kinase (MAGUK family)|calcium/calmodulin-dependent serine protein kinase membrane-associated guanylate kinase|protein lin-2 homolog|trinucleotide repeat containing 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASK, check out the CASK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CASK Antibody Picoband™ (A02468-2)
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No publications found for A02468-2
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