Product Info Summary
SKU: | A05259-1 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Caspase-5 CASP5 Antibody
SKU/Catalog Number
A05259-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Caspase-5 CASP5 Antibody (Catalog # A05259-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Caspase-5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Caspase-5 CASP5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05259-1)
Host
Rabbit
Contents
Caspase-5 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Caspase-5 antibody was raised against a 14 amino acid synthetic peptide from the center of human Caspase-5. The immunogen is located within amino acids 170 - 220 of Caspase-5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Depending on cell lines or tissues used, other cleavage products may be observed.
Reactive Species
A05259-1 is reactive to CASP5 in Human, Mouse
Applications
A05259-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
49.736kDa
Background of Caspase 5
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18. Caspase-5 can interact with caspase-1; both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1β. Expression of caspase-5 can be regulated by lipopolysaccharide (LPS) and IFN-gamma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Casp-5 antibody can be used for the detection of caspase-5 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of caspase-5 in Ramos cells with caspase-5 antibody at (A) 0.5
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Immunocytochemistry of caspase-5 in P815 cells with caspase-5 antibody at 2 μg/mL.
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Immunofluorescence of Caspase-5 in P815 cells with Caspase-5 antibody at 2 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For CASP5 (Source: Uniprot.org, NCBI)
Gene Name
CASP5
Full Name
Caspase-5
Weight
49.736kDa
Superfamily
peptidase C14A family
Alternative Names
CASP5; CASP-5; caspase 5, apoptosis-related cysteine peptidase; caspase 5, apoptosis-related cysteine protease; Caspase5; Caspase-5; EC 3.4.22; EC 3.4.22.58; ICE(rel)III; ICE(rel)-III; ICEREL-III; ICH3; ICH-3; MGC141966; Protease ICH-3; Protease TY; TY protease CASP5 ICE(rel)III, ICEREL-III, ICH-3 caspase 5 caspase-5|TY protease|caspase 5 variant X1|caspase 5, apoptosis-related cysteine peptidase|caspase 5, apoptosis-related cysteine protease|protease ICH-3|protease TY
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP5, check out the CASP5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase-5 CASP5 Antibody (A05259-1)
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Customer Reviews
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