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Product Info Summary
| SKU: | A00080-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IP, IF, ICC, WB |
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Product info
Product Name
Anti-Caspase-9 CASP9 Antibody
SKU/Catalog Number
A00080-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Caspase-9 CASP9 Antibody (Catalog # A00080-1). Tested in ELISA, WB, ICC, IF, IP applications. This antibody reacts with Human, Mouse.
Storage & Handling
Caspase-9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Caspase-9 CASP9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00080-1)
Host
Rabbit
Contents
Caspase-9 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-Caspase 9 antibody was raised against a peptide corresponding to 20 amino acids near the center of human Caspase 9. The immunogen is located within amino acids 290-340 of Caspase 9.
Cross-reactivity
Caspase-9 antibody is predicted to have no cross reactivity to other members in the caspase family.
Reactive Species
A00080-1 is reactive to CASP9 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
46.3 kDa
Background of CASP9
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00080-1 is guaranteed for ELISA, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1 μg/mL; ICC: 2- 5 μg/mL; IF: 5-20 μg/mL.
Antibody validated: Western Blot in human and mouse samples, Immunocytochemistry, Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: Caspase 9, 2071 (1 μg/mL), Caspase 9, A00080-1 (1 μg/mL) and beta-actin (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: : Caspase 9, A00080-1 (1 μg/mL)), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Mouse Cell Line
Loading: 15 μg of 3T3/NIH cell lysate.
Antibodies: : Caspase 9, A00080-1 (1 μg/mL)), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunofluorescence Validation of Caspase 9 in K562 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Caspase 9 with A00080-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
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Immunofluorescence Validation of Caspase 9 in HeLa Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Caspase 9 with A00080-1 at 5 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Immunocytochemistry Validation of Caspase 9 in HeLa Cells
Immunocytochemical analysis of HeLa cells using anti-Caspase 9 antibody (A00080-1) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Specific Publications For Anti-Caspase-9 CASP9 Antibody (A00080-1)
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