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- Table of Contents
CASP9 antibody (A00080-1) performs well in ELISA, IP, IF, ICC, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Immunofluorescence Validation of Caspase 9 in K562 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Caspase 9 with A00080-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Caspase 9 with A00080-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
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Anti-Caspase-9 CASP9 Antibody
CASP9 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | ELISA, IP, IF, ICC, WB |
Recommend Dilution
Application
Dilution
Species
WB: 1 μg/mL; ICC: 2- 5 μg/mL; IF: 5-20 μg/mL.
Antibody validated: Western Blot in human and mouse samples
Immunocytochemistry
Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Caspase-9 CASP9 Antibody
View all CASP9 Antibodies
View all CASP9 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Caspase-9 antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
Caspase-9 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-Caspase 9 antibody was raised against a peptide corresponding to 20 amino acids near the center of human Caspase 9. The immunogen is located within amino acids 290-340 of Caspase 9.
Cross-Reactivity
Caspase-9 antibody is predicted to have no cross reactivity to other members in the caspase family.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
46.3 kDa
Background of CASP9
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.
Cite This Product
Anti-Caspase-9 CASP9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00080-1)
Publications
Application:
Species:
Li QY,Hou CZ,Yang LP,Chu XL,Wang Y,Zhang P,Zhao Y. Study on the Mechanism of Ginseng in the Treatment of Lung Adenocarcinoma Based on Network Pharmacology. Evid Based Complement Alternat Med.2020 Jul 31;2020:2658795. doi:10.1155/2020/2658795.PMID:32802118
Species: Human
PMID: 32802118
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Product Promise
We promise Anti-Caspase-9 CASP9 Antibody performs excellently in relevant applications for ELISA, IP, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00080-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026