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Product Info Summary
| SKU: | M00080-5 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Chicken, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IP, IF, IHC, WB |
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Product info
Product Name
Anti-Caspase 9 CASP9 Monoclonal Antibody
SKU/Catalog Number
M00080-5
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Caspase 9 CASP9 Monoclonal Antibody catalog # M00080-5. Tested in IP, IF, IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase 9 CASP9 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00080-5)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
43891
Isotype
IgG1
Immunogen
Synthetic Peptide of Caspase 9
Reactive Species
M00080-5 is reactive to CASP9 in Chicken, Human, Mouse, Rat
Calculated molecular weight
46.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00080-5 is guaranteed for IP, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IP 1:200
IF 1:200
IHC 1:50-300
Validation Images & Assay Conditions
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Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000
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Western blot analysis of lysates from 1) Hela, 2) Jurkat, 3)3T3 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody was diluted at 1:10000, 37° 1hour. (Red) GAPDH Polyclonal Antibody was diluted at 1:5000 as loading control, 4° over night, secondary antibody was diluted at 1:10000, 37° 1hour.
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Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Caspase 9 Monoclonal Antibody (3-20) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, Caspase 9 Monoclonal Antibody (3-20) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunofluorescence analysis of Hela cell. 1, eIF2α Polyclonal Antibody (red) was diluted at 1:200 (4° overnight). Caspase 9 Monoclonal Antibody (3-20) (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
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1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200
Specific Publications For Anti-Caspase 9 CASP9 Monoclonal Antibody (M00080-5)
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