Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody

Caspase-9 antibody

Boster Bio Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody catalog # M00080-3. Tested in WB, IHC, ICC/IF, Flow Cytometry, IP applications. This antibody reacts with Human. Cited in 3 publication(s).

Product Info Summary

SKU: M00080-3
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

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Product Name

Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody

View all Caspase-9 Antibodies

SKU/Catalog Number

M00080-3

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody catalog # M00080-3. Tested in WB, IHC, ICC/IF, Flow Cytometry, IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00080-3)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AOC-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Caspase-9

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00080-3 is reactive to CASP9 in Human

Applications

M00080-3 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

46 kDa

Calculated molecular weight

46.281kDa

Background of Caspase-9

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CASP9 (Source: Uniprot.org, NCBI)

Gene Name

CASP9

Full Name

Caspase-9

Weight

46.281kDa

Superfamily

peptidase C14A family

Alternative Names

APAF-3APAF3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; CASP9; CASP-9; caspase 9, apoptosis-related cysteine peptidase; Caspase9; Caspase-9; EC 3.4.22.62; ICE-LAP6CASPASE-9c; ICE-like apoptotic protease 6; MCH6apoptosis-related cysteine protease CASP9 APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56 caspase 9 caspase-9|ICE-like apoptotic protease 6|apoptotic protease MCH-6|apoptotic protease activating factor 3|caspase 9, apoptosis-related cysteine peptidase|protein phosphatase 1, regulatory subunit 56

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CASP9, check out the CASP9 Infographic

CASP9 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CASP9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00080-3 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

In Vitro Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of the MCF-7 Human Breast Cancer Cell Line

In vivo?and?in vitro?evaluation of the cytotoxic effects of Photosan-loaded hollow silica nanoparticles on liver cancer

Chang C, Liu Sp, Fang Ch, He Rs, Wang Z, Zhu Yq, Jiang Sw. Oncol Lett. 2013 Sep;6(3):699-704. Epub 2013 Jul 8. Effects Of Matrine On The Proliferation Of Ht29 Human Colon Cancer Cells And Its Antitumor Mechanism.

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15 Customer Q&As for Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody

Question

I have attached the WB image, lot number and protocol we used for t-cell using anti-Caspase-9 Rabbit Monoclonal antibody M00080-3. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-28

Question

I was wanting to use your anti-Caspase-9 Rabbit Monoclonal antibody for IF for human t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human t-cell identification?

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

You can see on the product datasheet, M00080-3 anti-Caspase-9 Rabbit Monoclonal antibody has been validated for IP, IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-29

Question

Is this M00080-3 anti-Caspase-9 Rabbit Monoclonal antibody reactive to the isotypes of CASP9?

Verified Customer

Verified customer

Asked: 2019-10-24

Answer

The immunogen of M00080-3 anti-Caspase-9 Rabbit Monoclonal antibody is A synthesized peptide derived from human Caspase-9. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-10-24

Question

Can you help my question with product M00080-3, anti-Caspase-9 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-30

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00080-3 anti-Caspase-9 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-30

Question

My lab would like to test anti-Caspase-9 Rabbit Monoclonal antibody M00080-3 on human t-cell for research purposes, then I may be interested in using anti-Caspase-9 Rabbit Monoclonal antibody M00080-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

The products we sell, including anti-Caspase-9 Rabbit Monoclonal antibody M00080-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-12

Question

Is a blocking peptide available for product anti-Caspase-9 Rabbit Monoclonal antibody (M00080-3)?

Verified Customer

Verified customer

Asked: 2019-05-23

Answer

We do provide the blocking peptide for product anti-Caspase-9 Rabbit Monoclonal antibody (M00080-3). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-05-23

Question

We bought anti-Caspase-9 Rabbit Monoclonal antibody for IHC on kidney in a previous project. I am using human, and We intend to use the antibody for IF next. I am interested in examining kidney as well as cervix carcinoma erythroleukemia in our next experiment. Could give a recommendation on which antibody would work the best for IF?

O. Jackson

Verified customer

Asked: 2018-10-08

Answer

I have checked the website and datasheets of our anti-Caspase-9 Rabbit Monoclonal antibody and it seems that M00080-3 has been tested on human in both IHC and IF. Thus M00080-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-10-08

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-Caspase-9 Rabbit Monoclonal antibody M00080-3. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-08-30

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-08-30

Question

I see that the anti-Caspase-9 Rabbit Monoclonal antibody M00080-3 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-07-19

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-07-19

Question

Would M00080-3 anti-Caspase-9 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

J. Kulkarni

Verified customer

Asked: 2017-11-14

Answer

It shows on the product datasheet, M00080-3 anti-Caspase-9 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-11-14

Question

We are currently using anti-Caspase-9 Rabbit Monoclonal antibody M00080-3 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?

M. Patel

Verified customer

Asked: 2017-02-28

Answer

The anti-Caspase-9 Rabbit Monoclonal antibody (M00080-3) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-02-28

Question

Would anti-Caspase-9 Rabbit Monoclonal antibody M00080-3 work for IF with t-cell?

A. Mangal

Verified customer

Asked: 2016-10-26

Answer

According to the expression profile of t-cell, CASP9 is highly expressed in t-cell. So, it is likely that anti-Caspase-9 Rabbit Monoclonal antibody M00080-3 will work for IF with t-cell.

Boster Scientific Support

Answered: 2016-10-26

Question

We have seen staining in human adrenal tissue. Do you have any suggestions? Is anti-Caspase-9 Rabbit Monoclonal antibody supposed to stain adrenal tissue positively?

V. Thomas

Verified customer

Asked: 2016-05-19

Answer

Based on literature adrenal tissue does express CASP9. Based on Uniprot.org, CASP9 is expressed in adrenal tissue, t-cell, stomach cancer, kidney, eye lymph, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CASP9 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, and Lymph, Pubmed ID: 15489334
Kidney, Pubmed ID: 14702039
Stomach cancer, Pubmed ID: 9890966
T-cell, Pubmed ID: 8900201

Boster Scientific Support

Answered: 2016-05-19

Question

Do you have a BSA free version of anti-Caspase-9 Rabbit Monoclonal antibody M00080-3 available?

S. Miller

Verified customer

Asked: 2014-02-27

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-9 Rabbit Monoclonal antibody M00080-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-02-27

Question

I was wanting to use using your anti-Caspase-9 Rabbit Monoclonal antibody for formation of apoptosome studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

E. Rodriguez

Verified customer

Asked: 2013-07-23

Answer

I appreciate your inquiry. This M00080-3 anti-Caspase-9 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IP, IF, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2013-07-23

Order DetailsPrice
M00080-3

100μL

$370
M00080-3-30ul

30μL sample

$99
M00080-3-carrier-free

Carrier Free

$370

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M00080-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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