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- Table of Contents
CASP9 antibody (M00080) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 14 Publications
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Immunofluorescent analysis of HeLa cells treated with staurosporine, using Caspase-9 Antibody.
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Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody
CASP9 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
CASP9 antibody was positively detected by WB in:
human Hela whole cell, human K562 whole cell, human SiHa whole cell, human HEL whole cell, human Jurkat whole cell, human Jurkat whole cell, mouse NIH/3T3 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CASP9 antibody was positively detected by IHC in:
human liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CASP9 antibody was positively detected by ICC/IF in:
HeLa cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
Human
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody
View all CASP9 Antibodies
View all CASP9 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GEO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Caspase-9
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
46 kDa
Calculated Molecular Weight
46.3 kDa
Cite This Product
Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00080)
Protocols
Western Blot protocol for CASP9 antibody
Immunohistochemistry (IHC) protocol for CASP9 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CASP9 antibody
Publications
Application:
Species:
Promoting Drp1-Mediated Mitochondrial Division in Nickel Nanoparticles-Induced Reproductive Toxicity in GC-2 Cells
Published: 2025-12-25
Journal: Nanomaterials
Application: WB
Impact Factor: 4.30
Species: Mouse
PMID: 41511229
The effect of microRNA-145 on proliferation and apoptosis of cutaneous squamous cell carcinoma cells
Published: 2025-07-01
Journal: Discover Oncology
Application: WB
Impact Factor: 2.90
Species: Human
PMID: 40591050
Integrated network pharmacology, molecular docking and in vivo experiments to elucidate the extenuative mechanisms of ginseng polysaccharide against Toxoplasma gondii-induced testicular toxicity
Published: 2025-01-24
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 4.70
Species: Mouse
PMID: 39862631
Structural characteristics and anti-tumor effect of low molecular weight Dendrobium officinale polysaccharides by reconstructing tumor microenvironment
Published: 2024-06-20
Journal: Journal of Functional Foods
Application: WB
Impact Factor: 3.80
Species: Mouse
The Study of SRSF1 Regulates Abnormal Alternative Splicing of BCL2L11 and the Role in Refractory Acute Myeloid Leukemia
Published: 2024-06-12
Journal: CHEMOTHERAPY
Application: WB
Impact Factor: 2.00
Species: Human
PMID: 38763139
Mitochondrial Fission in Nickel Nanoparticle-Induced Reproductive Toxicity: An In Vitro GC-1 Cell Study
Published: 2024-04-17
Journal: Nanomaterials
Application: WB
Impact Factor: 4.40
Species: Mouse
PMID: 38668183
Dioscin induces osteosarcoma cell apoptosis by upregulating ROS-mediated P38 MAPK signaling
Published: 2022-11-19
Journal: DRUG DEVELOPMENT RESEARCH
Application: WB
Impact Factor: 5.00
Species: Human
PMID: 36401839
Preparation of Nereid oligopeptide and investigation of the mechanism underlying the induction of apoptosis in human lung cancer H1299 cells
Published: 2022-04-14
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 3.42
Species: Human
PMID: 35425994
Thiazolidinedione-Based Structure Modification of Celastrol Provides Thiazolidinedione-Conjugated Derivatives as Potent Agents against Non-Small-Cell Lung Cancer Cells through a Mitochondria-Mediated Apoptotic Pathway
Published: 2022-03-08
Journal: JOURNAL OF NATURAL PRODUCTS
Application: WB
Impact Factor: 4.80
Species: Human
PMID: 35255689
Transmembrane 4 L Six Family Member 1 Suppresses Hormone Receptor-–Positive, HER2-Negative Breast Cancer Cell Proliferation
Published: 2022-01-27
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 5.99
Species: Human
PMID: 35153775
Aconitine induces cell apoptosis via mitochondria and death receptor signaling pathways in hippocampus cell line
Published: 2022-01-05
Journal: RESEARCH IN VETERINARY SCIENCE
Application: WB
Impact Factor: 2.55
Species: Mouse
PMID: 35026629
Protective effects of pentadecapeptide derived from Cyclaina sinensis against cyclophosphamide-induced hepatotoxicity
Published: 2019-10-10
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Application: WB
Impact Factor: 2.71
Species: Mouse
PMID: 31607481
Wall shear stress promotes intimal hyperplasia through the paracrine H2O2-mediated NOX-AKT-SVV axis
Published: 2018-05-27
Journal: LIFE SCIENCES
Application: WB
Impact Factor: 3.23
Species: Rabbit,Human
PMID: 29847774
Cell cycle arrest and apoptosis of OVCAR-3 and MCF-7 cells induced by co-immobilized TNF-α plus IFN-γ on polystyrene and the role of p53 activation
Published: 2012-06-08
Journal: BIOMATERIALS
Application: WB
Impact Factor: 7.40
Species: Human
PMID: 22682938
Review
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Product Promise
We promise Anti-Caspase-9 CASP9 Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00080
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026