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Product Info Summary
| SKU: | A01307-3 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-CB2 Antibody (C-term)
SKU/Catalog Number
A01307-3
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-CB2 Antibody (C-term) (Catalog # A01307-3). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CB2 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01307-3)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-356 amino acids from the C-terminal region of human CB2.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01307-3 is reactive to CNR2 in Human
Calculated molecular weight
39.7 kDa
Background of CNR2
The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective, and pertussis toxin-sensitive manner. These proteins have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01307-3 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:25
Validation Images & Assay Conditions
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CB2 Antibody (C-term) western blot analysis in A431 cell line lysates (35ug/lane).This demonstrates the CB2 antibody detected the CB2 protein (arrow).
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Immunohistochemical analysis of paraffin-embedded R. brain section using CB2 Antibody(C-term). A01307-3 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded H. brain section using CB2 Antibody(C-term). A01307-3 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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CB2 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CB2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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CB2 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Flow cytometric analysis of Jurkat cells using CB2 Antibody (C-term) compared to an isotype control of rabbit IgG(blue). A01307-3 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Specific Publications For Anti-CB2 Antibody (C-term) (A01307-3)
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