Anti-Cbl-c Antibody

Product Name

Anti-Cbl-c Antibody

View all Cbl-c Antibodies

SKU/Catalog Number

A03693

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Cbl-c Antibody (Catalog # A03693). Tested in ELISA, IHC, IP, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Cbl-c Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03693)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal portion of Human Cbl-c.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03693 is reactive to CBLC in Human

Applications

A03693 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

52.456kDa

Background of Cbl-c

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Cbl-c is also known as signal transduction protein CBL-C, SH3-binding protein CBL-C, CBL-3, and RING finger protein 57. Cbl proteins are a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation. Cbl-c (a.k.a. Cbl-3) is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells (the other Cbl proteins are ubiquitously expressed). Cbl-c, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation. Cbl-c knock out mice show no obvious phenotype. Thus, the physiological role of Cbl-c is not known.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IHC: 1:500 - 1:3,000
IP: User optimized
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

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Cbl-c was detected in paraffin-embedded sections of a variety of tissues including multi-human, multi-brain and multi-cancer slides using rabbit anti-Cbl-c Antigen Affinity purified polyclonal antibody (Catalog # A03693) at 5 μg/mL. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.

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Cbl-c was detected in paraffin-embedded sections of ubuli in human kidney tissue using rabbit anti-Cbl-c Antigen Affinity purified polyclonal antibody (Catalog # A03693). Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.

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Western blot analysis of Cbl-c expression in transfected cell lysates (left panel). Lysates are from Hek 293T cells transfected with empty vector or with Cbl-c. The predicted size of Cbl-c is 52 kDa. Size markers in kDa are shown to the left of the panel. The right panel shows western blotting after first immunoprecipitating with Rabbit anti-Cbl-c followed by western blotting using a Goat anti-Cbl-c antibody. Cbl-c at 52KD was detected using rabbit anti-Cbl-c Antigen Affinity purified polyclonal antibody (Catalog # A03693). Personal Communication. Stan Lipkowitz, NCI, NIH, Bethesda, MD.

Gene/Protein Information For CBLC (Source: Uniprot.org, NCBI)

Alternative Names

Cas-Br-M (murine) ecotropic retroviral transforming sequence c; Cas-Br-M (murine) ectropic retroviral transforming sequence c; CBL3; CBL-3; CBL-SL; RING finger protein 57; RNF57SH3-binding protein CBL-3; SH3-binding protein CBL-C; signal transduction protein CBL-C CBLC CBL-3, CBL-SL, RNF57 Cbl proto-oncogene C E3 ubiquitin-protein ligase CBL-C|Cas-Br-M (murine) ecotropic retroviral transforming sequence c|Cas-Br-M (murine) ectropic retroviral transforming sequence c|Cbl proto-oncogene C, E3 ubiquitin protein ligase|Cbl proto-oncogene, E3 ubiquitin protein ligase C|RING finger protein 57|RING-type E3 ubiquitin transferase CBL-C|SH3-binding protein CBL-3|SH3-binding protein CBL-C|signal transduction protein CBL-C

For more info on CBLC, check out the CBLC Infographic

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In this infographic, you will see the following information for CBLC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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