Product Info Summary
SKU: | A04206-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CBX1/HP1 beta Antibody Picoband™
SKU/Catalog Number
A04206-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CBX1/HP1 Antibody Picoband™ catalog # A04206-2. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CBX1/HP1 beta Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04206-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CBX1/HP1 beta recombinant protein (Position: E55-N185).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04206-2 is reactive to CBX1 in Human, Mouse, Rat
Applications
A04206-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
21 kDa
Calculated molecular weight
21.418kDa
Background of CBX1
Chromobox protein homolog 1 is a protein that in humans is encoded by the CBX1 gene. This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunofluorescence, 5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CBX1/HP1 beta using anti-CBX1/HP1 beta antibody (A04206-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: huamn SW620 whole cell lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBX1/HP1 beta antigen affinity purified polyclonal antibody (Catalog # A04206-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBX1/HP1 beta at approximately 21 kDa. The expected band size for CBX1/HP1 beta is at 21 kDa.
Click image to see more details
Figure 2. IHC analysis of CBX1/HP1 beta using anti-CBX1/HP1 beta antibody (A04206-2).
CBX1/HP1 beta was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CBX1/HP1 beta Antibody (A04206-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CBX1/HP1 beta using anti-CBX1/HP1 beta antibody (A04206-2).
CBX1/HP1 beta was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CBX1/HP1 beta Antibody (A04206-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of CBX1/HP1 beta using anti-CBX1/HP1 beta antibody (A04206-2).
CBX1/HP1 beta was detected in a paraffin-embedded section of mouse intestines tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CBX1/HP1 beta Antibody (A04206-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of CBX1/HP1 beta using anti-CBX1/HP1 beta antibody (A04206-2).
CBX1/HP1 beta was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CBX1/HP1 beta Antibody (A04206-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IF analysis of CBX1/HP1 beta using anti-CBX1/HP1 beta antibody (A04206-2).
CBX1/HP1 beta was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CBX1/HP1 beta Antibody (A04206-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CBX1 (Source: Uniprot.org, NCBI)
Gene Name
CBX1
Full Name
Chromobox protein homolog 1
Weight
21.418kDa
Alternative Names
CBXM31HP1-BETA; chromobox homolog 1 (Drosophila HP1 beta); chromobox homolog 1 (HP1 beta homolog Drosophila ); chromobox homolog 1; chromobox protein homolog 1; Heterochromatin protein 1 homolog beta; heterochromatin protein 1-beta; heterochromatin protein p25 beta; Heterochromatin protein p25; HP1 beta homolog; HP1 beta; HP1Hsbeta; HP1Hs-beta; MOD1; Modifier 1 protein; p25beta CBX1 CBX, HP1-BETA, HP1Hs-beta, HP1Hsbeta, M31, MOD1, p25beta chromobox 1 chromobox protein homolog 1|HP1 beta homolog|chromobox homolog 1 (HP1 beta homolog Drosophila )|heterochromatin protein 1 homolog beta|heterochromatin protein 1-beta|heterochromatin protein p25 beta|modifier 1 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CBX1, check out the CBX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CBX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CBX1/HP1 beta Antibody Picoband™ (A04206-2)
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