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Product Info Summary
| SKU: | A17261 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Chicken |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-CCDC69 Antibody
SKU/Catalog Number
A17261
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CCDC69 Antibody (Catalog # A17261). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CCDC69 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CCDC69 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17261)
Host
Chicken
Contents
CCDC69 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgY
Immunogen
CCDC69 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CCDC69. The immunogen is located within amino acids 150 - 200 of CCDC69.
Reactive Species
A17261 is reactive to CCDC69 in Human, Mouse, Rat
Observed Molecular Weight
35 kDa
Calculated molecular weight
34.8 kDa
Background of CCDC69
The coiled-coil domain is a common protein motif that is often involved in protein oligomerization and is found in proteins such as transcription factors and intermediate filaments. The CCDC47 gene maps to chromosome 5 at 5q33.1. Little is known about this protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A17261 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-2 μg/mL; IF: 5-20 μg/mL; IHC: 1-5 μg/mL.
Antibody validated: Western Blot in human, mouse and ratsamples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Validation Images & Assay Conditions
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WB Validation in Human Cell Lines
Loading: 15 μg of lysate.
Antibodies: CCDC69 A17261, 1 μg/mL , 1 h incubation at RT in 8% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
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Western Blot Validation in Mouse Pancreas Tissue Lysate
Loading: 15 μg of lysates per lane.
Antibodies: CCDC69 A17261, 1 μg/mL, 1h incubation at RT in 8% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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Western Blot Validation in Rat Pancreas Lysate
Loading: 15 μg of lysates per lane.
Antibodies: CCDC69 A17261, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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Immunohistochemistry of CCDC69 in human lung tissue with CCDC69 antibody at 5 μg/mL.
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Immunofluorescence of CCDC69 in human lung tissue with CCDC69 antibody at 20 μg/mL.
Specific Publications For Anti-CCDC69 Antibody (A17261)
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