Anti-C-C chemokine receptor type 4 CCR4 Antibody

Boster Bio Anti-C-C chemokine receptor type 4 CCR4 Antibody catalog # PA1517. Tested in WB applications. This antibody reacts with Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PA1517
Size: 100μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-C-C chemokine receptor type 4 CCR4 Antibody

See all Ccr4 products

SKU/Catalog Number

PA1517

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-C-C chemokine receptor type 4 CCR4 Antibody catalog # PA1517. Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-C-C chemokine receptor type 4 CCR4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1517)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of mouse CCR4 (346-360aa, SYTQSTVDHDFRDAL), identical to the related rat sequence.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1517 is reactive to Ccr4 in Mouse, Rat

Applications

PA1517 is guaranteed for WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For Ccr4 (Source: Uniprot.Org, NCBI)

Uniprot ID

P51680

Gene ID

12773

Gene Name

Ccr4

Full Name

C-C chemokine receptor type 4

Weight

41.408kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

C-C chemokine receptor type 4; C-C CKR-4; CC-CKR-4HGCN:14099; CCR4; CCR-4; CD194 antigen; CD194; chemokine (C-C motif) receptor 4; chemokine (C-C) receptor 4; ChemR13; CKR4MGC88293; CMKBR4k5-5; K5-5 Ccr4|C-C CKR-4, CHEMR1, Cmkbr, Cmkbr4, LESTR, Sdf1r|chemokine (C-C motif) receptor 4|C-C chemokine receptor type 4|chemokine (C-C) receptor 4|fusin|leukocyte-expressed seven-transmembrane-domain receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ccr4, check out the Ccr4 Infographic

Ccr4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ccr4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1517 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhao C, Bu X, Wang W, Ma T, Ma H. Plos One. 2014 Jan 8;9(1):E85058. Doi: 10.1371/Journal.Pone.0085058. Ecollection 2014. Gec-Derived Sfrp5 Inhibits Wnt5A-Induced Macrophage Chemotaxis And Activation.

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4 Customer Q&As for Anti-C-C chemokine receptor type 4 CCR4 Antibody

Question

We are currently using anti-CCR4 antibody PA1517 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

The anti-CCR4 antibody (PA1517) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-14

Question

I am looking for using your anti-CCR4 antibody for immune response studies. Has this antibody been tested with western blotting on rat thymus tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-10-23

Answer

Thanks for your inquiry. This PA1517 anti-CCR4 antibody is validated on rat thymus tissue, tissue lysate, spleen tissue, hepa whole cell lysate. It is guaranteed to work for WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-10-23

Question

Our lab were happy with the WB result of your anti-CCR4 antibody. However we have seen positive staining in spleen cell membrane using this antibody. Is that expected? Could you tell me where is CCR4 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-04-25

Answer

Based on literature, spleen does express CCR4. Generally CCR4 expresses in cell membrane. Regarding which tissues have CCR4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Spleen, Pubmed ID: 7642634

Boster Scientific Support

Answered: 2019-04-25

Question

We have been able to see staining in mouse leukocyte. Are there any suggestions? Is anti-CCR4 antibody supposed to stain leukocyte positively?

D. Johnson

Verified customer

Asked: 2018-01-24

Answer

According to literature leukocyte does express CCR4. According to Uniprot.org, CCR4 is expressed in leukocyte, spleen, blood, among other tissues. Regarding which tissues have CCR4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Spleen, Pubmed ID: 7642634

Boster Scientific Support

Answered: 2018-01-24

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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PA1517
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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