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Product Info Summary
| SKU: | PA1201 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CCR6/CCR6 Antibody Picoband®
SKU/Catalog Number
PA1201
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CCR6/CCR6 Antibody catalog # PA1201. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CCR6/CCR6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1201)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CCR6, different from the related rat and mouse sequences by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1201 is reactive to CCR6 in Human, Mouse, Rat
Observed Molecular Weight
120 kDa
Calculated molecular weight
42.5 kDa
Background of CCR6
CCR6, also termed CKRL3, encodes a 369-amino acid polypeptide with greatest similarity to the family of alpha-chemokine-binding receptors. Unlike most chemokine receptor genes, it is encoded by more than 1 exon. Weakly expressed as a 4-kb transcript in spleen, lymph nodes, peripheral blood lymphocytes and appendix, CCR6 gene is located at 6q27. As the receptor for MIP-3-alpha, its activation leads to phospholipase C-dependent intracellular Ca (2+) mobilization. Additionally, CCR6 are markedly upregulated in psoriasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1201 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Liver Tissue, SW620 Cell, CEM Cell
Validation Images & Assay Conditions
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Anti-CCR6 antibody, PA1201, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: SW620 Cell Lysate
Lane 3: CEM Cell Lysate
Specific Publications For Anti-CCR6/CCR6 Antibody Picoband® (PA1201)
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Customer Q&As
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3 Customer Q&As for Anti-CCR6/CCR6 Antibody Picoband®
Question
Is this PA1201 anti-CCR6/CCR6 antibody reactive to the isotypes of CCR6?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
The immunogen of PA1201 anti-CCR6/CCR6 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human CCR6(358-374aa RQTSETADNDNASSFTM), different from the related rat and mouse sequences by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-04
Question
Does anti-CCR6/CCR6 antibody PA1201 work on zebrafish WB with pancreas?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
Our lab technicians have not validated anti-CCR6/CCR6 antibody PA1201 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-CCR6/CCR6 antibody PA1201 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish pancreas in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-21
Question
We are currently using anti-CCR6/CCR6 antibody PA1201 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-22
Answer
The anti-CCR6/CCR6 antibody (PA1201) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-22
