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Product Info Summary
| SKU: | M00390 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CCR7 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00390
BM4218 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CCR7 Rabbit Monoclonal Antibody catalog # M00390. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CCR7 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00390)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CFO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CCR7
Reactive Species
M00390 is reactive to CCR7 in Human, Mouse, Rat
Observed Molecular Weight
43 kDa
Calculated molecular weight
42.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00390 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: human MCF-7 whole cell, human K562 whole cell, rat spleen tissue, rat lung tissue, rat C6 whole cell, mouse spleen tissue, mouse lung tissue
IHC: human spleen tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of CCR7 using anti-CCR7 antibody (M00390).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat spleen tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse spleen tissue lysates,
Lane 7: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR7 antigen affinity purified monoclonal antibody (Catalog # M00390) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR7 at approximately 43 kDa. The expected band size for CCR7 is at 43 kDa.
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Immunohistochemical analysis of paraffin-embedded human spleen, using CCR7 Antibody.
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Immunofluorescent analysis of Hela cells, using CCR7 Antibody .
Specific Publications For Anti-CCR7 Rabbit Monoclonal Antibody (M00390)
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6 Customer Q&As for Anti-CCR7 Rabbit Monoclonal Antibody
Question
I see that the anti-CCR7 Rabbit Monoclonal antibody M00390 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-04-13
Question
We are currently using anti-CCR7 Rabbit Monoclonal antibody M00390 for mouse tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
The anti-CCR7 Rabbit Monoclonal antibody (M00390) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-21
Question
Will anti-CCR7 Rabbit Monoclonal antibody M00390 work for IF with blood?
Verified Customer
Verified customer
Asked: 2019-01-22
Answer
According to the expression profile of blood, CCR7 is highly expressed in blood. So, it is likely that anti-CCR7 Rabbit Monoclonal antibody M00390 will work for IF with blood.
Boster Scientific Support
Answered: 2019-01-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-CCR7 Rabbit Monoclonal antibody M00390. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-01-22
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-22
Question
Is this M00390 anti-CCR7 Rabbit Monoclonal antibody reactive to the isotypes of CCR7?
M. Jha
Verified customer
Asked: 2015-11-11
Answer
The immunogen of M00390 anti-CCR7 Rabbit Monoclonal antibody is A synthesized peptide derived from human CCR7. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-11-11
Question
I have attached the WB image, lot number and protocol we used for blood using anti-CCR7 Rabbit Monoclonal antibody M00390. Please let me know if you require anything else.
C. Krishna
Verified customer
Asked: 2015-04-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-09
