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Product Info Summary
| SKU: | M03674-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD162 Monoclonal Antibody
SKU/Catalog Number
M03674-1
BM5618 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD162 Monoclonal Antibody catalog # M03674-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD162 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03674-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEHF-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD162 A SLe (x) -type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
Reactive Species
M03674-1 is reactive to SELPLG in Human
Observed Molecular Weight
120 kDa
Calculated molecular weight
43.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03674-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:40
Positive Control
WB: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of CD162 expression in Jurkat cell lysate.
Specific Publications For Anti-CD162 Monoclonal Antibody (M03674-1)
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Customer Q&As
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3 Customer Q&As for Anti-CD162 Monoclonal Antibody
Question
Is there a BSA free version of anti-CD162 Monoclonal antibody M03674-1 available?
Verified Customer
Verified customer
Asked: 2018-05-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD162 Monoclonal antibody M03674-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-22
Question
We are currently using anti-CD162 Monoclonal antibody M03674-1 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
J. Baker
Verified customer
Asked: 2014-10-20
Answer
The anti-CD162 Monoclonal antibody (M03674-1) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-20
Question
Will anti-CD162 Monoclonal antibody M03674-1 work for WB with erythroleukemia?
B. Mitchell
Verified customer
Asked: 2013-11-05
Answer
According to the expression profile of erythroleukemia, SELPLG is highly expressed in erythroleukemia. So, it is likely that anti-CD162 Monoclonal antibody M03674-1 will work for WB with erythroleukemia.
Boster Scientific Support
Answered: 2013-11-05
