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Product Info Summary
| SKU: | M03674 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-CD162 (Selectin P Ligand) SELPLG Monoclonal Antibody
View all PSGL-1/CD162 Antibodies
SKU/Catalog Number
M03674
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CD162 (Selectin P Ligand) SELPLG Monoclonal Antibody (Catalog # M03674). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CD162 (Selectin P Ligand) SELPLG Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03674)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: PSGL1/1601
Isotype
IgG1, kappa
Immunogen
Recombinant human SELPLG (CD162) protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M03674 is reactive to SELPLG in Human
Applications
M03674 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
43.201kDa
Background of PSGL-1/CD162
CD162 glycoprotein functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Spleen stained with Anti-CD162 Monoclonal Antibody (PSGL1/1601).
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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-CD162 Monoclonal Antibody (PSGL1/1601).
Protein Target Info & Infographic
Gene/Protein Information For SELPLG (Source: Uniprot.org, NCBI)
Gene Name
SELPLG
Full Name
P-selectin glycoprotein ligand 1
Weight
43.201kDa
Alternative Names
CD162 antigen; CD162; P-selectin glycoprotein ligand 1; PSGL1; PSGL-1; PSGL-1cutaneous lymphocyte-associated associated antigen; selectin P ligandCLA; SELPLG SELPLG CD162, CLA, PSGL-1, PSGL1 selectin P ligand P-selectin glycoprotein ligand 1|cutaneous lymphocyte-associated associated antigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SELPLG, check out the SELPLG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SELPLG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD162 (Selectin P Ligand) SELPLG Monoclonal Antibody (M03674)
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No publications found for M03674
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