Product Info Summary
SKU: | A00282 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SREBF1 Antibody
SKU/Catalog Number
A00282
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SREBF1 Antibody (Catalog # A00282). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
SREBF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-SREBF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00282)
Host
Rabbit
Contents
SREBF1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
SK7
Isotype
IgG
Immunogen
SREBF1 antibody was raised against a 17 amino acid peptide near the center of human SREBF1. The immunogen is located within amino acids 450 - 500 of SREBF1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
SREBF1 antibody is human and mouse reactive. At least three isoforms of SREBF1 are known to exist. SREBF1 antibody is predicted not to cross-react with SREBF2.
Reactive Species
A00282 is reactive to SREBF1 in Human, Mouse
Applications
A00282 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
121.675kDa
Background of SREBP1
The sterol regulatory element binding transcription factor 1 (SREBF1) is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis (1). The related protein SREBF2 also binds SRE1 and activates transcription in an additive fashion to SREBF1 (2). SREBF1 is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1 (3). The SREBF1 proteins are important in the regulation of genes involved in lipid metabolism, while SREBF2 has been more closely associated with cholesterol synthesis and accumulation (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SREBF1 antibody can be used for detection of SREBF1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SREBF1 in Daudi cell lysate with SREBF1 antibody at 1 μg/ml.
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Immunohistochemistry of SREBF1 in human spleen tissue with SREBF1 antibody at 5 μg/mL.
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Immunofluorescence of SREBF1 in human spleen tissue with SREBF1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For SREBF1 (Source: Uniprot.org, NCBI)
Gene Name
SREBF1
Full Name
Sterol regulatory element-binding protein 1
Weight
121.675kDa
Superfamily
SREBP family
Alternative Names
BHLHD1; Class D basic helix-loop-helix protein 1; SREBP 1c; SREBP-1; SREBP1bHLHd1SREBP-1c; sterol regulatory element binding protein-1; sterol regulatory element binding transcription factor 1; sterol regulatory element-binding protein 1; Sterol regulatory element-binding transcription factor 1 SREBF1 HMD, IFAP2, SREBP1, bHLHd1 sterol regulatory element binding transcription factor 1 sterol regulatory element-binding protein 1|class D basic helix-loop-helix protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SREBF1, check out the SREBF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SREBF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SREBF1 Antibody (A00282)
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No publications found for A00282
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Recommended Resources
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