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Product Info Summary
| SKU: | M05056 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-CD168 HMMR Rabbit Monoclonal Antibody
SKU/Catalog Number
M05056
BM4527 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD168 HMMR Rabbit Monoclonal Antibody catalog # M05056. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD168 HMMR Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05056)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FFA-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD168
Reactive Species
M05056 is reactive to HMMR in Human, Mouse, Rat
Observed Molecular Weight
85 kDa
Calculated molecular weight
84.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05056 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Positive Control
IHC: Human stomach cancer tissue, Human lung large cell cancer tissue, Human Hodgkin's lymphoma tissue, human testis tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded Human stomach cancer, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Antibody at 1:50 dilution.
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Western blot analysis of CD168 expression in LnCaP cell lysate.
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Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded human testis, using CD168 Antibody.
Specific Publications For Anti-CD168 HMMR Rabbit Monoclonal Antibody (M05056)
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Customer Q&As
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3 Customer Q&As for Anti-CD168 HMMR Rabbit Monoclonal Antibody
Question
I see that the anti-CD168 Rabbit Monoclonal antibody M05056 works with WB, what is the protocol used to produce the result images on the product page?
S. Baker
Verified customer
Asked: 2017-10-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-10-23
Question
Would anti-CD168 Rabbit Monoclonal antibody M05056 work for WB with cervix carcinoma?
N. Anderson
Verified customer
Asked: 2014-12-01
Answer
According to the expression profile of cervix carcinoma, HMMR is highly expressed in cervix carcinoma. So, it is likely that anti-CD168 Rabbit Monoclonal antibody M05056 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2014-12-01
Question
Will M05056 anti-CD168 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Krishna
Verified customer
Asked: 2013-08-07
Answer
As indicated on the product datasheet, M05056 anti-CD168 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-08-07
