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Product Info Summary
| SKU: | A06184 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CD1E Antibody
SKU/Catalog Number
A06184
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD1E Antibody catalog # A06184. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD1E Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06184)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human CD1E. AA range:321-370
Reactive Species
A06184 is reactive to CD1E in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
43.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06184 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of K562 cells using CD1E Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysate from K562 cells, using CD1E Antibody.
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Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100
Specific Publications For Anti-CD1E Antibody (A06184)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-CD1E Antibody
Question
I have a question about product A06184, anti-CD1E antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06184 anti-CD1E antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-02
Question
Is this A06184 anti-CD1E antibody reactive to the isotypes of CD1E?
V. Miller
Verified customer
Asked: 2019-07-16
Answer
The immunogen of A06184 anti-CD1E antibody is Synthesized peptide derived from the C-terminal region of human CD1E. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-16
Question
We are currently using anti-CD1E antibody A06184 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
V. Zhao
Verified customer
Asked: 2019-05-10
Answer
The anti-CD1E antibody (A06184) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-10
Question
See attached the WB image, lot number and protocol we used for thymus using anti-CD1E antibody A06184. Please let me know if you require anything else.
K. Krishna
Verified customer
Asked: 2017-02-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-02-08
