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- Table of Contents
Cd28 antibody (M00065-1) performs well in IHC-P and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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IHC analysis of CD28 using anti-CD28 antibody (M00065-1).
CD28 was detected in paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti-CD28 Antibody (M00065-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
CD28 was detected in paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti-CD28 Antibody (M00065-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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Anti- CD28 Monoclonal Antibody
Cd28 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Mouse | IHC-P |
Tested Applications
Cd28 antibody was positively detected by IHC in:
mouse thymus tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Product Information
Product Name
Anti- CD28 Monoclonal Antibody
View all CD28 Antibodies
View all CD28 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
PBS, pH 7.0. Contains no stabilizers or preservatives
Clonality
Monoclonal
Clone Number
AGOA-3
Isotype
Mouse IgG1, κ
Immunogen
A20 cells expressing mouse CD28 and a recombinant mouse CD28-Ig fusion protein
Cross-Reactivity
No cross reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
42 kDa
Calculated Molecular Weight
25.2 kDa
Background of Cd28
CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co- stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). The CD28 gene is mapped to 2q33-q34. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily.
Cite This Product
Anti- CD28 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00065-1)
Protocols
Immunohistochemistry (IHC) protocol for Cd28 antibody
Publications
Application:
Species:
The involvement of Th1 cell differentiation in the anti-tumor effect of purified polysaccharide from Sanghuangporus vaninii in colorectal cancer via multi-omics analysis
Published: 2023-03-07
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: IHC
Impact Factor: 8.20
Species: Mouse
PMID: 36889619
Combination of Chidamide-Mediated Epigenetic Modulation with Immunotherapy: Boosting Tumor Immunogenicity and Response to PD-1/PD-L1 Blockade
Published: 2021-08-16
Journal: ACS Applied Materials & Interfaces
Application: FC
Impact Factor: 9.23
Species: Mouse
PMID: 34433253
PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules
Published: 2018-11-09
Journal: EBioMedicine
Application: IHC
Impact Factor: 6.18
Species: Mouse
PMID: 30420298
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Product Promise
We promise Anti- CD28 Monoclonal Antibody performs excellently in relevant applications for IHC-P. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00065-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026