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Product Info Summary
| SKU: | M00065-1 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Mouse |
| Host: | Mouse |
| Application: | IHC-P |
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Product info
Product Name
Anti- CD28 Monoclonal Antibody
SKU/Catalog Number
M00065-1
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti- CD28 Monoclonal Antibody catalog # M00065-1. Tested in IHC-P applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti- CD28 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00065-1)
Host
Mouse
Contents
PBS, pH 7.0. Contains no stabilizers or preservatives
Clonality
Monoclonal
Clone Number
AGOA-3
Isotype
Mouse IgG1, κ
Immunogen
A20 cells expressing mouse CD28 and a recombinant mouse CD28-Ig fusion protein
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M00065-1 is reactive to Cd28 in Mouse
Observed Molecular Weight
42 kDa
Calculated molecular weight
25.2 kDa
Background of Cd28
CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co- stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). The CD28 gene is mapped to 2q33-q34. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00065-1 is guaranteed for IHC-P Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse
Positive Control
IHC: mouse thymus tissue
Validation Images & Assay Conditions
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IHC analysis of CD28 using anti-CD28 antibody (M00065-1).
CD28 was detected in paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti-CD28 Antibody (M00065-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Specific Publications For Anti- CD28 Monoclonal Antibody (M00065-1)
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