CD28 Antibodies

AND CD28 ELISA kits, CD28 Proteins

Many biological assays use antibodies to detect CD28, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with CD28 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop CD28 antibodies...

We validate the specificity of these antibodies to CD28 by testing them on tissues known to express CD28 positively and negatively. Browse below to find the CD28 antibody that suites your experiment. We have 41 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for CD28? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the CD28 IHC Guide

CD28 Infographic by Boster Bio

All CD28 Products

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Product

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Specificity

Applications

Price

Anti-ICOS Antibody Picoband®

1 Citations

IHC analysis of ICOS using anti-ICOS antibody (A00291-2)

Human, Mouse, Rat

ELISA, IHC, WB

$370
Cat # A00291-2
100 μg/vial

Human CD28 ELISA Kit PicoKine®

Human

ELISA

$499
Cat # EK1429
96 wells/kit, with removable strips.

Anti- CD28 Monoclonal Antibody

3 Citations

IHC analysis of CD28 using anti-CD28 antibody (M00065-1)

Mouse

IHC-P

$299
Cat # M00065-1
100μg/vial

Anti-PINK1 Antibody Picoband®

8 Citations

Western blot analysis of PINK1 using anti-PINK1 antibody (A00201-2)

IF analysis of PINK1 using anti-PINK1 antibody (A00201-2)

Flow Cytometry analysis of 293T cells using anti-PINK1 antibody (A00201-2)

Human

ELISA, Flow Cytometry, IF, ICC, WB

$370
Cat # A00201-2
100 μg/vial

Human B7-1/CD80 ELISA Kit PicoKine®

1 Citations

Human

ELISA

$499
Cat # EK0707
96 wells/kit, with removable strips.

Anti-CD80 Antibody Picoband®

1 Citations

Western blot analysis of CD80 using anti-CD80 antibody (A00196-1)

IHC analysis of CD80 using anti-CD80 antibody (A00196-1)

Human, Mouse, Rat

ELISA, IHC, WB

$370
Cat # A00196-1
100 μg/vial

Human B7-1/CD80 PicoKine® Quick ELISA Kit

Human

ELISA

$499
Cat # FEK0707
96 wells/kit, with removable strips.

Anti-CD80 Antibody

WB Validation in Human Raji Cells
Loading: 15 μg of lysate Antibodies: CD80, A00196, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST

WB Validation in Human Thymus
Loading: 15 μg of lysate Antibodies: CD80, A00196, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST

WB Validation in Human Tonsil
Loading: 15 μg of lysate Antibodies: CD80, A00196, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST

Human, Mouse, Rat

ELISA, IF, WB

$481
Cat # A00196
0.1 mg

Anti-Cd80 Antibody Picoband®

3 Citations

Western blot analysis of Cd80 using anti-Cd80 antibody (A00196-3)

Flow Cytometry analysis of ANA-1 cells using anti-Cd80 antibody (A00196-3)

Human, Mouse

ELISA, Flow Cytometry, WB

$370
Cat # A00196-3
100 μg/vial

Anti-CD80 Monoclonal Antibody [8G12]

Immunocytochemistry of CD80 in transfected HEK293 cells with CD80 antibody at 1 μg/mL

Immunofluorescence of CD80 in transfected HEK293 cells with CD80 antibody at 2 μg/mL

Human

ELISA, Flow Cytometry, IF, IHC-P, ICC

$481
Cat # M00196-1
0.1 mg

Anti-TLR3 Antibody Picoband®

3 Citations

Western blot analysis of TLR3 using anti-TLR3 antibody (A00197)

Human, Mouse, Rat

ELISA, WB

$370
Cat # A00197
100 μg/vial

Anti-TLR1 Antibody Picoband®

IHC analysis of TLR1 using anti-TLR1 antibody (A00429-2)

Human, Mouse, Rat

ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB

$370
Cat # A00429-2
100 μg/vial

Anti-Toll-like receptor 1 TLR1 Antibody Picoband®

Western blot analysis of TLR1 using anti-TLR1 antibody (A00429-3)

IHC analysis of TLR1 using anti-TLR1 antibody (A00429-3)

Flow Cytometry analysis of A549 cells using anti-TLR1 antibody (A00429-3)

Human, Rat

ELISA, Flow Cytometry, IHC, WB

$370
Cat # A00429-3
100 μg/vial

Anti-CD28 Antibody

Western blot analysis of CD28 in human spleen tissue lysate with CD28 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide

Human

ELISA, IF, IHC, WB

$481
Cat # A00065
0.1 mg

Anti-FGFR3 Antibody Picoband®

Western blot analysis of FGFR3 using anti-FGFR3 antibody (A00200-3)

IF analysis of FGFR3 using anti-FGFR3 antibody (A00200-3)

Flow Cytometry analysis of 293T cells using anti-FGFR3 antibody (A00200-3)

Human

Flow Cytometry, IF, ICC, WB

$370
Cat # A00200-3
100 μg/vial

Anti-TLR9 Antibody Picoband®

Western blot analysis of TLR9 using anti-TLR9 antibody (A00198-3)

IHC analysis of TLR9 using anti-TLR9 antibody (A00198-3)

Human, Rat

IHC, WB

$370
Cat # A00198-3
100 μg/vial

Anti-CD28 Reference Antibody (lulizumab)

The purity of Anti-CD28 Reference Antibody (lulizumab)is more than 100%

Human

ELISA, Flow Cytometry, Functional Assay, Kinetics

$486
Cat # M00065-5
100 μg

Anti-CD28 Reference Antibody (FR104)

The purity of Anti-CD28 Reference Antibody (FR104)is more than 98

Human

ELISA, Flow Cytometry, Functional Assay, Kinetics

$486
Cat # M00065-4
100 μg

Anti-CD28 Reference Antibody (Theralizumab)

The purity of Anti-CD28 Reference Antibody (Theralizumab)is more than 98

Human

ELISA, Flow Cytometry, Functional Assay, Kinetics

$486
Cat # M00065-3
100 μg

Anti-CD28 Antibody Picoband®

Western blot analysis of CD28 using anti-CD28 antibody (A00065-3)

Flow Cytometry analysis of HEL cells using anti-CD28 antibody (A00065-3)

Human

ELISA, Flow Cytometry, WB

$370
Cat # A00065-3
100 μg/vial

Anti-MRC2 / CD280 Reference Antibody (Quark Patent Anti-Endo180)

The purity of Anti-MRC2/CD280 Reference Antibody (Quark Patent Anti-Endo180)is more than 95%

Human

ELISA, Flow Cytometry, Functional Assay, Kinetics

$486
Cat # M04070-4
100 μg

Anti-MRC2 / CD280 Reference Antibody (Quark patent anti-ENDO 180)

Human

ELISA, Flow Cytometry, Functional Assay, Kinetics

$486
Cat # M04070-3
100 μg

Anti-MRC2 / CD280 Reference Antibody (Copenhagen Rigshospitalet patent anti-uPARAP)

The purity of Anti-MRC2/CD280 Reference Antibody (Copenhagen Rigshospitalet patent anti-uPARAP)is more than 95%

Human

ELISA, Flow Cytometry, Functional Assay, Kinetics

$486
Cat # M04070-2
100 μg

Anti-TLR3 / CD283 Reference Antibody (CNTO5429)

The purity of Anti-TLR3/CD283 Reference Antibody (CNTO5429)is more than 95%

Human

ELISA, Flow Cytometry, Functional Assay, Kinetics

$486
Cat # M00197
100 μg

Anti-CD28L / CD80 Reference Antibody (galiximab)

The purity of Anti-CD28L/CD80 Reference Antibody (galiximab)is more than 95

Human

ELISA, Flow Cytometry, Functional Assay, Kinetics

$486
Cat # M00196-5
100 μg

Anti-TLR2 / CD282 Reference Antibody (tomaralimab)

The purity of Anti-TLR2/CD282 Reference Antibody (tomaralimab)is more than 100%

Human, Mouse

ELISA, Flow Cytometry, Functional Assay, Kinetics

$486
Cat # M00131-2
100 μg

Anti-CD80 Antibody Picoband®

Western blot analysis of CD80 using anti-CD80 antibody (PA1549)

Western blot analysis of CD80 using anti-CD80 antibody (PA1365)

Human

$370
Cat # PA1365
100 μg/vial

CD28 (NM_006139) Human Over-expression Lysate

Human

$628
Cat # LS000940
20 µg, 100 µg

Anti-CD80/B7 1 Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature

Western blot analysis of CD80 expression in Raji cell lysate

Human

IHC, WB

$370
Cat # M00196
100 μl

Mouse B7-1/CD80 Recombinant Protein

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Mouse CD80 is coated at 2 ug/ml (100 ul/well) can bind Mouse CTLA4 (CAT# PROTP09793-1) in a Functional ELISA assay

SDS-PAGE

$265
Cat # PROTQ00609
Starting from 10ug

Human recombinant CD28 ECD protein, AF

Human

Cell Culture

$77
Cat # PROTP10747-2
5ug,20ug,100ug

Human recombinant TMIGD2/CD28H protein

SDS-PAGE, Bioactivity

$261
Cat # PROTQ96BF3-1
Starting from 10ug

CD28 (NM_006139) Human Recombinant Protein

$1296
Cat # PROTP10747
20 µg

Anti-Toll-like receptor 3 TLR3 Antibody

Western blot analysis of TLR3 in Daudi cell lysate with TLR3 antibody at (A) 1 and (B) 2 μg/mL

Immunofluorescence of TLR3 in mouse spleen tissue with TLR3 antibody at 20 μg/ml

WB Validation in Human Cells
Loading: 15 μg of lysate Antibodies: TLR3, A00197-2, 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST

Human, Mouse, Rat

ELISA, IF, IHC-P, WB

$481
Cat # A00197-2
0.1 mg

Anti-TMIGD2 Monoclonal Antibody [8A1]

Western blot analysis of TMIGD2 in overexpressing HEK293 cells TMIGD2 antibody at 0

Immunocytochemistry of TMIGD2 in transfected HEK293 cells with TMIGD2 antibody at 2 μg/mL

Human

ELISA, Flow Cytometry, IF, IHC-P, ICC, WB

$481
Cat # M11415
0.1 mg

Anti-Toll-like receptor 1 TLR1 Antibody

Western blot analysis of TLR1 in mouse spleen lysate with TLR1 antibody at (A) 1, (B) 2, and (C) 4 μg/mL

Immunohistochemistry of TLR1 in mouse spleen tissue with TLR1 antibody at 10 μg/mL

Immunofluorescence of TLR1 in Mouse Spleen tissue

Human, Mouse

ELISA, IF, IHC-P, WB

$481
Cat # A00429
0.1 mg

Anti-Toll-like receptor 9 TLR9 Antibody

Western blot analysis of TLR9 in Jurkat cell lysate with TLR9 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide

Immunocytochemistry of TLR9 in Jurkat cells with TLR9 antibody at 2 μg/mL

Immunofluorescence of TLR9 in Mouse Spleen tissue with TLR9 antibody at 10 μg/mL

Human, Mouse, Rat

ELISA, IF, ICC, WB

$481
Cat # A00198-2
0.1 mg

Anti-Toll-like receptor 9 TLR9 Antibody

Western blot analysis of TLR9 in mouse spleen cell lysate with TLR9 antibody at (A) 0

Immunohistochemistry of TLR9 in mouse spleen cells with TLR9 antibody at 2 μg/mL

Immunofluorescence of TLR9 in mouse spleen cells with TLR9 antibody at 10 μg/mL

Human, Mouse

ELISA, IF, IHC-P, WB

$481
Cat # A00198-1
0.1 mg

Anti-Toll-like receptor 2 TLR2 Antibody

Western blot analysis of TLR2 in A20 cell lysates with TLR2 antibody at 1 μg/mL in the presence (A) and absence (B) of its blocking peptide

Immunohistochemical staining of human spleen cells using TLR2 antibody at 2 μg/mL

Immunofluorescence of TLR2 in Human Spleen tissue with TLR2 antibody at 10 μg/mL

Human, Mouse

ELISA, IF, IHC-P, WB

$481
Cat # A00131-1
0.1 mg

Anti-TLR2 Antibody

Human

$299
Cat # M00131-1
50 µl

Human TLR2 Recombinant Protein

SDS-PAGE

$350
Cat # PROTO60603
Starting from 10ug

CD28 Overview

Facts about T-cell-specific surface glycoprotein CD28.

CD28 3D structure. Source: alphafold

T-cell-specific surface glycoprotein CD28 (CD28)

Involved in T-cell activation, the induction of cell proliferation and cytokine production and marketing of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933).

Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). .

Gene Information

Human
Gene Name:	CD28
Uniprot:	P10747
Entrez:	940

Family

Belongs to:

No superfamily

Alternative Names

CD28 antigen (Tp44); CD28 antigen; CD28 molecule; CD28; MGC138290; T-cell-specific surface glycoprotein CD28; Tp44

Molecular Weight

Mass (kDA):
25.066 kDA

Genomic Context


Human

Location:	2q33.2
Sequence:	2; NC_000002.12 (203706475..203739756)

Tissue Specificity

Expressed in T-cells and plasma cells, but not in less mature B-cells.

Subcellular Localizations

Membrane; Single-pass type I membrane protein.; [Isoform 3]: Cell surface.

Diseases

Neoplasms
Autoimmune Reaction
Autoimmune Diseases
Hiv Infections
Malignant Neoplasms
Inflammation
Immunologic Deficiency Syndromes
Tissue Adhesions
Autoimmunity
Decreased Immunologic Activity [pe]
Arthritis
Rheumatoid Arthritis
Infective Disorder

Leukemia
Graft-vs-host Disease
Lymphoma
Virus Diseases
Cytomegalovirus Infections
Carcinoma
Lupus Erythematosus, Systemic

Interacting Proteins

PIK3R1

Pathways

Cell Activation
T Cell Activation
Cell Proliferation
Immune Response
Secretion
Cytokine Production
T Cell Proliferation
Pathogenesis
Cell Death
Cell Cycle
Cell Differentiation
Aging
Cytokine Secretion

Lymphocyte Activation
Senescence
Localization
T Cell Costimulation
Cell Growth
Viral Replication
Cell Division

PTMs

Phosphorylation
Dephosphorylation
Glycosylation
Acetylation
Cleavage
Methylation
Ubiquitination
Demethylation
Reduction
Myristoylation

Phosphorothioation
Nitration
Prenylation
Ribosylation
Biotinylation
Oxidation
Nitrosylation
Gpi Anchoring
Cysteinylation
Farnesylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CD28

References

PMID: 2825196 by Aruffo A., et al. Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system.

PMID: 2162892 by Lee K.P., et al. The genomic organization of the CD28 gene. Implications for the regulation of CD28 mRNA expression and heterogeneity.

7 Publications of Boster CD28 Antibodies & ELISA Kits

PubMed ID: 10.1089/scd.2019.0039, A Study of the Immunoregulatory Function of TLR3 and TLR4 on Mesenchymal Stem Ce...
PubMed ID: 10.1021/acsami.1c08290, Combination of Chidamide-Mediated Epigenetic Modulation with Immunotherapy: Boos...
PubMed ID: 33607154, Liao L,Huang L,Wei X,Yin L,Wei X,Li T.Bioinformatic and biochemical studies of f...
PubMed ID: 33607154, Liao L,Huang L,Wei X,Yin L,Wei X,Li T.Bioinformatic and biochemical studies of f...
PubMed ID: 33537094, Chen X,Gao A,Zhang F,Yang Z,Wang S,Fang Y,Li J,Wang J,Shi W,Wang L, Zheng Y,Sun ...
PubMed ID: 32801828, Feng S,Ju L,Shao Z,Grzanna M,Jia L,Liu M.Therapeutic Effect of C-C Chemokine Rec...
PubMed ID: 22728711, Du Hy, Dong Lh, Zhao Bj, Fu J, Wang Qq, Chen F, Ou L, Li N, Sun X, Tang Zm, Song...

*More publications can be found for each product on its corresponding product page