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Product Info Summary
| SKU: | M04405 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD3D Rabbit Monoclonal Antibody
SKU/Catalog Number
M04405
BM4818 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD3D Rabbit Monoclonal Antibody catalog # M04405. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD3D Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04405)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IDE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD3D
Reactive Species
M04405 is reactive to CD3D in Human
Observed Molecular Weight
21 kDa
Calculated molecular weight
18.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04405 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: human Jurkat whole cell, human MOLT-4 whole cell
IHC: human spleen tissue
ICC/IF: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of CD3D using anti-CD3D antibody (M04405).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human MOLT-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3D antigen affinity purified monoclonal antibody (Catalog # M04405) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD3D at approximately 21 kDa. The expected band size for CD3D is at 19 kDa.
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Immunohistochemical analysis of paraffin-embedded human spleen, using CD3D Antibody.
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Immunofluorescent analysis of Jurkat cells, using CD3D Antibody.
Specific Publications For Anti-CD3D Rabbit Monoclonal Antibody (M04405)
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5 Customer Q&As for Anti-CD3D Rabbit Monoclonal Antibody
Question
Please see the WB image, lot number and protocol we used for thymus using anti-CD3D Rabbit Monoclonal antibody M04405. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-30
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-CD3D Rabbit Monoclonal antibody M04405. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-13
Question
Will M04405 anti-CD3D Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Johnson
Verified customer
Asked: 2018-11-12
Answer
As indicated on the product datasheet, M04405 anti-CD3D Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-12
Question
Our lab want to know about to test anti-CD3D Rabbit Monoclonal antibody M04405 on human thymus for research purposes, then I may be interested in using anti-CD3D Rabbit Monoclonal antibody M04405 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-07-09
Answer
The products we sell, including anti-CD3D Rabbit Monoclonal antibody M04405, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-09
Question
We are currently using anti-CD3D Rabbit Monoclonal antibody M04405 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-14
Answer
The anti-CD3D Rabbit Monoclonal antibody (M04405) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-14
