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Product Info Summary
| SKU: | MA1016 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, ICC |
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Product info
Product Name
Anti-CD4 Antibody (Monoclonal, CA-4)
SKU/Catalog Number
MA1016
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD4 Antibody (Monoclonal, CA-4) catalog # MA1016. Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD4 Antibody (Monoclonal, CA-4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1016)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
CA-4
Isotype
Mouse IgG1
Immunogen
CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1016 is reactive to CD4 in Human
Applications
MA1016 is guaranteed for Flow Cytometry, IHC, ICC Boster Guarantee
Observed Molecular Weight
25 kDa
Calculated molecular weight
51.111kDa
Background of CD4
CD stands for "cluster of differentiation"; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T,gp55]. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Flow Cytometry analysis of human PBMC cells using anti-CD4 antibody (MA1016).
Overlay histogram showing human PBMC cells stained with MA1016 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD4 Antibody (MA1016,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CD4 (Source: Uniprot.org, NCBI)
Gene Name
CD4
Full Name
T-cell surface glycoprotein CD4
Weight
51.111kDa
Alternative Names
CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 CD4 CD4mut CD4 molecule T-cell surface glycoprotein CD4|CD4 antigen (p55)|CD4 receptor|T-cell surface antigen T4/Leu-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD4, check out the CD4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD4 Antibody (Monoclonal, CA-4) (MA1016)
Hello CJ!
MA1016 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients
Zhao N, Li X, He X, Qiu Y, Zhu L, Qi F. J Gene Med. 2013 Oct;15(10):366-74. Doi: 10.1002/Jgm.2739. Interleukin-15 Gene Therapy And The Mammalian Target Of Rapamycin Inhibitor Everolimus Inhibit The Growth Of Metastatic Breast Cancer.
Wang X, Shi L, Tu Q, Wang H, Zhang H, Wang P, Zhang L, Huang Z, Zhao F, Luan H, Wang X. Int J Nanomedicine. 2015 Jan 6;10:347-55. Doi: 10.2147/Ijn.S71245. Ecollection 2015. Treating Cutaneous Squamous Cell Carcinoma Using 5-Aminolevulinic Acid Pol...
Xu Y, Tan X, Wang D, Wang W, Li Y, Wu M, Chen S, Wu Y, Tan C. Int J Mol Med. 2015 Jun;35(6):1501-10. Doi: 10.3892/Ijmm.2015.2154. Epub 2015 Mar 24. Elevated Survivin Expression In Peripheral Blood Mononuclear Cells Is Central To Collateral Formati...
Wu Y, Wang Y, Qi X, Zhang P, Zhang C, Zhang W. Nephron Exp Nephrol. 2014;128(1-2):46-56. Doi: 10.1159/000366446. Epub 2014 Nov 5. Increased Macrophage Activation Inhibited By Tacrolimus In The Kidney Of Diabetic Rats.
Chen X, Wan J, Liu J, Xie W, Diao X, Xu J, Zhu B, Chen Z. Lung Cancer. 2010 Sep;69(3):348-54. Doi: 10.1016/J.Lungcan.2009.11.013. Increased Il-17-Producing Cells Correlate With Poor Survival And Lymphangiogenesis In Nsclc Patients.
Ouyang W, An T, Guo D, Wu S, Tien P. Aids Res Hum Retroviruses. 2013 Mar;29(3):613-20. Doi: 10.1089/Aid.2012.0059. Epub 2013 Jan 11. The Potent Human Immunodeficiency Virus Type 1 (Hiv-1) Entry Inhibitor Hr212 Blocks Formation Of The Envelope Glyc...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
17 Customer Q&As for Anti-CD4 Antibody (Monoclonal, CA-4)
Question
My question regarding product MA1016, anti-CD4 antibody (Monoclonal, CA-4). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1016 anti-CD4 antibody (Monoclonal, CA-4), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-05-04
Question
We are currently using anti-CD4 antibody (Monoclonal, CA-4) MA1016 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
The anti-CD4 antibody (Monoclonal, CA-4) (MA1016) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-20
Question
Our lab want to know about using your anti-CD4 antibody (Monoclonal, CA-4) for cell adhesion studies. Has this antibody been tested with western blotting on human pbmc cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
I appreciate your inquiry. This MA1016 anti-CD4 antibody (Monoclonal, CA-4) is tested on human pbmc cells. It is guaranteed to work for Flow Cytometry, IHC, ICC in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-17
Question
We have tried in the past anti-CD4 antibody (Monoclonal, CA-4) for ICC on leukocyte in a previous experiment. I am using human, and We want to use the antibody for IHC next. We are interested in examining leukocyte as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
I looked at the website and datasheets of our anti-CD4 antibody (Monoclonal, CA-4) and it appears that MA1016 has been tested on human in both ICC and IHC. Thus MA1016 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-18
Question
Would anti-CD4 antibody (Monoclonal, CA-4) MA1016 work for Flow Cytometry with blood?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
According to the expression profile of blood, CD4 is highly expressed in blood. So, it is likely that anti-CD4 antibody (Monoclonal, CA-4) MA1016 will work for Flow Cytometry with blood.
Boster Scientific Support
Answered: 2019-09-26
Question
My colleagues were content with the WB result of your anti-CD4 antibody (Monoclonal, CA-4). However we have observed positive staining in liver cell membrane using this antibody. Is that expected? Could you tell me where is CD4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
From what I have seen in literature, liver does express CD4. Generally CD4 expresses in cell membrane. Regarding which tissues have CD4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1425921
Brain, Pubmed ID: 9074930
Liver, Pubmed ID: 19159218
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-08-22
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-CD4 antibody (Monoclonal, CA-4) MA1016. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-27
Question
See attached the WB image, lot number and protocol we used for blood using anti-CD4 antibody (Monoclonal, CA-4) MA1016. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-04
Question
Do you have a BSA free version of anti-CD4 antibody (Monoclonal, CA-4) MA1016 available?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD4 antibody (Monoclonal, CA-4) MA1016 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-02-21
Question
Is this MA1016 anti-CD4 antibody (Monoclonal, CA-4) reactive to the isotypes of CD4?
L. Parker
Verified customer
Asked: 2018-09-03
Answer
The immunogen of MA1016 anti-CD4 antibody (Monoclonal, CA-4) is CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-03
Question
I was wanting to use your anti-CD4 antibody (Monoclonal, CA-4) for Flow Cytometry for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2018-01-30
Answer
You can see on the product datasheet, MA1016 anti-CD4 antibody (Monoclonal, CA-4) has been validated for Flow Cytometry, IHC, ICC on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-30
Question
Would MA1016 anti-CD4 antibody (Monoclonal, CA-4) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-12-25
Answer
You can see on the product datasheet, MA1016 anti-CD4 antibody (Monoclonal, CA-4) as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-25
Question
Would anti-CD4 antibody (Monoclonal, CA-4) MA1016 work on primate IHC with liver?
W. Krishna
Verified customer
Asked: 2016-09-06
Answer
Our lab technicians have not tested anti-CD4 antibody (Monoclonal, CA-4) MA1016 on primate. You can run a BLAST between primate and the immunogen sequence of anti-CD4 antibody (Monoclonal, CA-4) MA1016 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-09-06
Question
I see that the anti-CD4 antibody (Monoclonal, CA-4) MA1016 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
R. Jha
Verified customer
Asked: 2016-07-21
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-07-21
Question
We have seen staining in human leukocyte. Any tips? Is anti-CD4 antibody (Monoclonal, CA-4) supposed to stain leukocyte positively?
D. Jha
Verified customer
Asked: 2016-04-25
Answer
From what I have seen in literature leukocyte does express CD4. From what I have seen in Uniprot.org, CD4 is expressed in leukocyte, brain, thymus, pancreas, blood, liver, among other tissues. Regarding which tissues have CD4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1425921
Brain, Pubmed ID: 9074930
Liver, Pubmed ID: 19159218
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2016-04-25
Question
I am interested in to test anti-CD4 antibody (Monoclonal, CA-4) MA1016 on human blood for research purposes, then I may be interested in using anti-CD4 antibody (Monoclonal, CA-4) MA1016 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Zhang
Verified customer
Asked: 2015-10-22
Answer
The products we sell, including anti-CD4 antibody (Monoclonal, CA-4) MA1016, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-10-22
Question
Is a blocking peptide available for product anti-CD4 antibody (Monoclonal, CA-4) (MA1016)?
A. Krishna
Verified customer
Asked: 2013-03-22
Answer
We do provide the blocking peptide for product anti-CD4 antibody (Monoclonal, CA-4) (MA1016). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-03-22
