Anti-CD47 Rabbit Monoclonal Antibody

Product Name

Anti-CD47 Rabbit Monoclonal Antibody

View all CD47 Antibodies

SKU/Catalog Number

M00360

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CD47 Rabbit Monoclonal Antibody catalog # M00360. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD47 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00360)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

D-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CD47

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00360 is reactive to CD47 in Human, Mouse, Rat

Applications

M00360 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

305 kDa

Calculated molecular weight

35.214kDa

Background of CD47

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

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Western blot analysis of extracts of NIH/3T3 cell lysate, using CD47 antibody.

Gene/Protein Information For CD47 (Source: Uniprot.org, NCBI)

Alternative Names

antigen identified by monoclonal 1D8; Antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen; CD47 glycoprotein; CD47 molecule; CD47; IAP; IAPintegrin associated protein; Integrin-associated protein; leukocyte surface antigen CD47; MER6integrin-associated signal transducer; OA3; Protein MER6; Rh-related antigen CD47 IAP, MER6, OA3 CD47 molecule leukocyte surface antigen CD47|CD47 antigen (Rh-related antigen, integrin-associated signal transducer)|CD47 glycoprotein|Rh-related antigen|antigen identified by monoclonal antibody 1D8|antigenic surface determinant protein OA3|integrin associated protein|integrin-associated signal transducer

For more info on CD47, check out the CD47 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD47: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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