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Product Info Summary
| SKU: | M03913-1 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC |
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Product info
Product Name
Anti-CD6 Monoclonal Antibody
SKU/Catalog Number
M03913-1
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CD6 Monoclonal Antibody (Catalog # M03913-1). Tested in IF, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CD6 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03913-1)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: C6/372 + 3F7B5
Isotype
IgG1, kappa + IgG1, kappa
Immunogen
Human recombinant CD6 protein (C6/372); Human rheumatoid synovial T cell line ST-1 (3F7B5)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M03913-1 is reactive to CD6 in Human
Applications
M03913-1 is guaranteed for IF, IHC Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
71.801kDa
Background of CD6
CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular scavenger receptor cysteine-rich (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-CD6 Monoclonal Antibody (C6/372+3F7B5).
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Immunofluorescence staining of MOLT-4 cells using Anti-CD6 Monoclonal Antibody (C6/372+3F7B5) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
Protein Target Info & Infographic
Gene/Protein Information For CD6 (Source: Uniprot.org, NCBI)
Gene Name
CD6
Full Name
T-cell differentiation antigen CD6
Weight
71.801kDa
Alternative Names
CD6 antigenFLJ44171; CD6 molecule; CD6; T12; Tp120; TP120T-cell differentiation antigen CD6 CD6 TP120 CD6 molecule T-cell differentiation antigen CD6|CD6 antigen|T12
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD6, check out the CD6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD6 Monoclonal Antibody (M03913-1)
Hello CJ!
No publications found for M03913-1
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Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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13 Customer Q&As for Anti-CD6 Monoclonal Antibody
Question
Does anti-CD6 Monoclonal antibody M03913-1 work for IHC with lymph node?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
According to the expression profile of lymph node, CD6 is highly expressed in lymph node. So, it is likely that anti-CD6 Monoclonal antibody M03913-1 will work for IHC with lymph node.
Boster Scientific Support
Answered: 2020-02-24
Question
We are currently using anti-CD6 Monoclonal antibody M03913-1 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
The anti-CD6 Monoclonal antibody (M03913-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-07
Question
Is a blocking peptide available for product anti-CD6 Monoclonal antibody (M03913-1)?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
We do provide the blocking peptide for product anti-CD6 Monoclonal antibody (M03913-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-06
Question
I see that the anti-CD6 Monoclonal antibody M03913-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-06
Question
Will anti-CD6 Monoclonal antibody M03913-1 work on dog IHC with lymph node?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
Our lab technicians have not validated anti-CD6 Monoclonal antibody M03913-1 on dog. You can run a BLAST between dog and the immunogen sequence of anti-CD6 Monoclonal antibody M03913-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog lymph node in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-23
Question
I was wanting to use your anti-CD6 Monoclonal antibody for IHC for human lymph node on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lymph node identification?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
As indicated on the product datasheet, M03913-1 anti-CD6 Monoclonal antibody has been validated for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymph node in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-08
Question
Would M03913-1 anti-CD6 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-03
Answer
As indicated on the product datasheet, M03913-1 anti-CD6 Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-03
Question
Is this M03913-1 anti-CD6 Monoclonal antibody reactive to the isotypes of CD6?
Verified Customer
Verified customer
Asked: 2018-08-14
Answer
The immunogen of M03913-1 anti-CD6 Monoclonal antibody is Human recombinant CD6 protein (C6/372); Human rheumatoid synovial T cell line ST-1 (3F7B5). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-14
Question
My question regarding product M03913-1, anti-CD6 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-08-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03913-1 anti-CD6 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-01
Question
Do you have a BSA free version of anti-CD6 Monoclonal antibody M03913-1 available?
W. Jones
Verified customer
Asked: 2017-11-30
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD6 Monoclonal antibody M03913-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-30
Question
We are interested in to test anti-CD6 Monoclonal antibody M03913-1 on human lymph node for research purposes, then I may be interested in using anti-CD6 Monoclonal antibody M03913-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-04
Answer
The products we sell, including anti-CD6 Monoclonal antibody M03913-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-04
Question
See below the WB image, lot number and protocol we used for lymph node using anti-CD6 Monoclonal antibody M03913-1. Please let me know if you require anything else.
Z. Johnson
Verified customer
Asked: 2014-02-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-02-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph node using anti-CD6 Monoclonal antibody M03913-1. Let me know if you need anything else.
N. Banerjee
Verified customer
Asked: 2013-04-01
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-04-01
