Mouse CD5L/CT-2 PicoKine™ Quick ELISA Kit

Mouse CD5L / CT 2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Cd5l in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: FEK1414
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay Time: 90 Minutes

Product Name

Mouse CD5L/CT-2 PicoKine™ Quick ELISA Kit

See all Cd5l products

SKU/Catalog Number

FEK1414

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse CD5L / CT 2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Cd5l in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse CD5L/CT-2 PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1414)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-V352

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1414 is reactive to Cd5l in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK1414 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog numberDescription QuantityVolume
FEK1414-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK1414-STMouse Cd5l Standard 2 10 ng/tube
FEK1414-CMouse Cd5l Antibody Cocktail 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)01563126251,2502,5005,00010,000
O.D.0.0040.1310.2090.4240.7471.2941.9962.436

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2011789494020719354821
Standard deviation13.26110.91375.4417.38131.58414.6
CV (%)6.6%6.2%7.6%8.4%6.8%8.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Cd5l in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 120123623222222213.556.1%
Sample 21789179518751980185977.284.1%
Sample 349404445482548344761187.963.9%
*number of samples for each test n=16.

Gene/Protein Information For Cd5l (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9QWK4

Gene ID

11801

Gene Name

Cd5l

Full Name

CD5 antigen-like

Weight

38.863kDa

Alternative Names

AIM; API6; apoptosis inhibitor 6; CD5 antigen-like (scavenger receptor cysteine rich family); CD5 antigen-like; CD5 molecule-like; CD5L; CT-2; IgM-associated peptide; PRO229; Spalpha; SP-alpha Cd5l|1/6, A, AAC-, AAC-11, AI047839, AIM, Api6, CT2, Pdp, Sp, Sp-alpha, mAIM|CD5 antigen-like|CD5 antigen-like|AIM/Spalpha|CT-2|Pdp 1/6|apoptosis inhibitor 6|apoptosis inhibitor expressed by macrophages

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cd5l, check out the Cd5l Infographic

Cd5l infographic

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In this infographic you will see the following information for Cd5l: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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